Matches in SemOpenAlex for { <https://semopenalex.org/work/W1900763607> ?p ?o ?g. }
- W1900763607 endingPage "2124" @default.
- W1900763607 startingPage "2118" @default.
- W1900763607 abstract "To understand the relationship between the primary structure and function of varicella-zoster virus thymidine kinase (VZV TK; EC 2.7.1.21), we established rapid screening and phenotypic selection of mutant VZV TK genes in TK-deficient Escherichia coli C600 by using a constitutive pKK223-3 expression plasmid. In this screening system, mutant TK genes generated by random mutagenesis were identified by the sensitivity of E. coli-expressing VZV TKs to 5-bromo-2'-deoxyuridine and 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl) uracil. Twenty-four mutant clones with amino acid substitutions were isolated, and their nucleotide sequence and enzymatic activities were determined. Of the 24 clones, 20 had single amino acid substitutions, 2 clones had double amino acid substitutions, and 1 clone had triple amino acid substitutions. In 17 cases of single amino acid substitution, six mutations led to lost enzyme activity, and four of these six mutations centered in the ATP-binding site. The other 11 mutations resulted in reduction of both TK and thymidylate kinase activities or only thymidylate kinase activity and were located in scattered positions in the VZV TK gene, although 5 mutations showed a tendency to cluster in the region between positions 251 and 260." @default.
- W1900763607 created "2016-06-24" @default.
- W1900763607 creator A5021336597 @default.
- W1900763607 creator A5039096844 @default.
- W1900763607 creator A5044330314 @default.
- W1900763607 creator A5065219194 @default.
- W1900763607 creator A5078409676 @default.
- W1900763607 date "1992-04-01" @default.
- W1900763607 modified "2023-09-25" @default.
- W1900763607 title "Random mutagenesis of the thymidine kinase gene of varicella-zoster virus" @default.
- W1900763607 cites W124504601 @default.
- W1900763607 cites W1497005879 @default.
- W1900763607 cites W1514278476 @default.
- W1900763607 cites W1530288812 @default.
- W1900763607 cites W1545962383 @default.
- W1900763607 cites W1552049113 @default.
- W1900763607 cites W1582808591 @default.
- W1900763607 cites W1606628486 @default.
- W1900763607 cites W1968982209 @default.
- W1900763607 cites W1974303276 @default.
- W1900763607 cites W1975304761 @default.
- W1900763607 cites W1988453706 @default.
- W1900763607 cites W1991292465 @default.
- W1900763607 cites W1994768832 @default.
- W1900763607 cites W1997285921 @default.
- W1900763607 cites W1997497266 @default.
- W1900763607 cites W2000098766 @default.
- W1900763607 cites W2003996277 @default.
- W1900763607 cites W2006643920 @default.
- W1900763607 cites W2013200026 @default.
- W1900763607 cites W2013627201 @default.
- W1900763607 cites W2013892901 @default.
- W1900763607 cites W2017122550 @default.
- W1900763607 cites W2017525552 @default.
- W1900763607 cites W2022002272 @default.
- W1900763607 cites W2025703152 @default.
- W1900763607 cites W2035273962 @default.
- W1900763607 cites W2045360603 @default.
- W1900763607 cites W2045761974 @default.
- W1900763607 cites W2049695588 @default.
- W1900763607 cites W2063466305 @default.
- W1900763607 cites W2069982799 @default.
- W1900763607 cites W2083236946 @default.
- W1900763607 cites W2086494981 @default.
- W1900763607 cites W2096485985 @default.
- W1900763607 cites W2114240169 @default.
- W1900763607 cites W2119559171 @default.
- W1900763607 cites W2137562741 @default.
- W1900763607 cites W2142922283 @default.
- W1900763607 cites W2158422971 @default.
- W1900763607 cites W2160978219 @default.
- W1900763607 cites W2167248608 @default.
- W1900763607 cites W2415772247 @default.
- W1900763607 cites W2460690294 @default.
- W1900763607 cites W4302944574 @default.
- W1900763607 cites W2326787353 @default.
- W1900763607 doi "https://doi.org/10.1128/jvi.66.4.2118-2124.1992" @default.
- W1900763607 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/289003" @default.
- W1900763607 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1312622" @default.
- W1900763607 hasPublicationYear "1992" @default.
- W1900763607 type Work @default.
- W1900763607 sameAs 1900763607 @default.
- W1900763607 citedByCount "23" @default.
- W1900763607 countsByYear W19007636072013 @default.
- W1900763607 countsByYear W19007636072015 @default.
- W1900763607 crossrefType "journal-article" @default.
- W1900763607 hasAuthorship W1900763607A5021336597 @default.
- W1900763607 hasAuthorship W1900763607A5039096844 @default.
- W1900763607 hasAuthorship W1900763607A5044330314 @default.
- W1900763607 hasAuthorship W1900763607A5065219194 @default.
- W1900763607 hasAuthorship W1900763607A5078409676 @default.
- W1900763607 hasBestOaLocation W19007636071 @default.
- W1900763607 hasConcept C104317684 @default.
- W1900763607 hasConcept C123321153 @default.
- W1900763607 hasConcept C143065580 @default.
- W1900763607 hasConcept C153911025 @default.
- W1900763607 hasConcept C159047783 @default.
- W1900763607 hasConcept C16318435 @default.
- W1900763607 hasConcept C22744801 @default.
- W1900763607 hasConcept C2522874641 @default.
- W1900763607 hasConcept C2776147627 @default.
- W1900763607 hasConcept C2776159415 @default.
- W1900763607 hasConcept C2776694085 @default.
- W1900763607 hasConcept C2780456651 @default.
- W1900763607 hasConcept C2781196997 @default.
- W1900763607 hasConcept C501734568 @default.
- W1900763607 hasConcept C515207424 @default.
- W1900763607 hasConcept C54355233 @default.
- W1900763607 hasConcept C55493867 @default.
- W1900763607 hasConcept C86803240 @default.
- W1900763607 hasConceptScore W1900763607C104317684 @default.
- W1900763607 hasConceptScore W1900763607C123321153 @default.
- W1900763607 hasConceptScore W1900763607C143065580 @default.
- W1900763607 hasConceptScore W1900763607C153911025 @default.
- W1900763607 hasConceptScore W1900763607C159047783 @default.
- W1900763607 hasConceptScore W1900763607C16318435 @default.
- W1900763607 hasConceptScore W1900763607C22744801 @default.
- W1900763607 hasConceptScore W1900763607C2522874641 @default.