Matches in SemOpenAlex for { <https://semopenalex.org/work/W1900817811> ?p ?o ?g. }
- W1900817811 endingPage "370" @default.
- W1900817811 startingPage "356" @default.
- W1900817811 abstract "Analysis of the cellular/molecular basis of the early steps of hematopoietic proliferation and differentiation is hindered by the rarity of hematopoietic progenitors and stem cells (HP/HSC). The intensive efforts devoted to the development of purification methods for early HP and HSC, although initially largely unsuccessful, have recently provided a high level of HP/HSC yield and/or recovery. The methodology developed by our group, recently improved, provides not only virtually complete purification, but also abundant recovery of early HP/HSC such as colony forming units granulocyte/erythroid/macrophage/megakaryocyte (CFU-GEMM), burst forming units erythroid (BFU-E), CFU granulocyte/macrophage (CFU-GM)/CFU blast cells (CFU-B), and long-term culture initiating cells (LTC-IC) from adult peripheral and cord blood (CB). We have also developed a serum-free liquid suspension culture for unilineage erythroid (E), granulocytic (G) or monocytic (M) differentiation of stringently purified HP/HSC. These culture systems allow sequential collection and cellular/molecular analysis of discrete populations of hematopoietic cells at a homogenous stage of differentiation specifically along a unilineage pathway. These experimental tools have been utilized to investigate cellular/molecular mechanisms underlying early hematopoiesis. The transcription factor (TF) GATA-1 is considered to be the “master” gene of erythropoiesis. In highly purified HP/HSC undergoing E or GM differentiation, GATA-1 expression is characterized initiaiiy by proliferation-dependent activation and at later stages by sustained expression in the E pathway and suppression in the GM pathway. Hypothetically, similar on/off switches of lineage-restricted TF may underlie the binary fate decisions of early HP differentiation. The expression and modulation of hematopoi-etic growth factor receptors (HGFR) in early hematopoiesis have been extensively analyzed. The results suggest a model of transactivation cascade for HGFR such as interleukin 6 receptor (IL-6R), IL-3R, GM colony stimulating factor receptor (GM-CSFR), and erythropoietin receptor (EpR), whereby each HGF upmodulates the R(s) for distal-acting HGF(s). Finally, we have investigated the effect of HGF on reactivation of hemoglobin F (HbF) in clono-genic or liquid suspension serum-free culture of purified adult HP. The results suggest that c-kit ligand (KL) plays a key role in the reactivation of HbF synthesis in adult life, and IL-3/GM-CSF potentiate this effect at low KL level. The KL-induced HbF reactivation is seemingly related to an enhanced proliferation of early E progenitors in their differentiation pathway." @default.
- W1900817811 created "2016-06-24" @default.
- W1900817811 creator A5008684425 @default.
- W1900817811 creator A5012596350 @default.
- W1900817811 creator A5021060698 @default.
- W1900817811 creator A5027329662 @default.
- W1900817811 creator A5038981750 @default.
- W1900817811 creator A5042442375 @default.
- W1900817811 creator A5050041073 @default.
- W1900817811 creator A5055619996 @default.
- W1900817811 creator A5066695968 @default.
- W1900817811 creator A5083113596 @default.
- W1900817811 date "1993-01-01" @default.
- W1900817811 modified "2023-10-05" @default.
- W1900817811 title "Stringently purified human hematopoietic progenitors/stem cells: Analysis of cellular/molecular mechanisms underlying early hematopoiesis" @default.
- W1900817811 cites W1484655458 @default.
- W1900817811 cites W1584828020 @default.
- W1900817811 cites W1585188298 @default.
- W1900817811 cites W1592018386 @default.
- W1900817811 cites W172498270 @default.
- W1900817811 cites W1836124358 @default.
- W1900817811 cites W1964895030 @default.
- W1900817811 cites W1966100365 @default.
- W1900817811 cites W1966144382 @default.
- W1900817811 cites W1966482743 @default.
- W1900817811 cites W1973035870 @default.
- W1900817811 cites W1973312385 @default.
- W1900817811 cites W1975331230 @default.
- W1900817811 cites W1977380444 @default.
- W1900817811 cites W2000246573 @default.
- W1900817811 cites W2000367099 @default.
- W1900817811 cites W2008698247 @default.
- W1900817811 cites W2010519150 @default.
- W1900817811 cites W2014045848 @default.
- W1900817811 cites W2016191371 @default.
- W1900817811 cites W2016433042 @default.
- W1900817811 cites W2018552733 @default.
- W1900817811 cites W2022963870 @default.
- W1900817811 cites W2027562868 @default.
- W1900817811 cites W2027761072 @default.
- W1900817811 cites W2027802649 @default.
- W1900817811 cites W2031179453 @default.
- W1900817811 cites W2031991725 @default.
- W1900817811 cites W2037695813 @default.
- W1900817811 cites W2040735196 @default.
- W1900817811 cites W2042021736 @default.
- W1900817811 cites W2049423476 @default.
- W1900817811 cites W2066790739 @default.
- W1900817811 cites W2068499309 @default.
- W1900817811 cites W2075973589 @default.
- W1900817811 cites W2076287231 @default.
- W1900817811 cites W2077813077 @default.
- W1900817811 cites W2085326817 @default.
- W1900817811 cites W2086565185 @default.
- W1900817811 cites W2086793358 @default.
- W1900817811 cites W2087831434 @default.
- W1900817811 cites W2094869608 @default.
- W1900817811 cites W2096205803 @default.
- W1900817811 cites W2099584527 @default.
- W1900817811 cites W2105695958 @default.
- W1900817811 cites W2106045733 @default.
- W1900817811 cites W2111759041 @default.
- W1900817811 cites W2120056767 @default.
- W1900817811 cites W2137696432 @default.
- W1900817811 cites W2138993111 @default.
- W1900817811 cites W2148459495 @default.
- W1900817811 cites W2148580930 @default.
- W1900817811 cites W2149604229 @default.
- W1900817811 cites W2150379132 @default.
- W1900817811 cites W2150951349 @default.
- W1900817811 cites W2155681299 @default.
- W1900817811 cites W216302928 @default.
- W1900817811 cites W2180300767 @default.
- W1900817811 cites W2266106978 @default.
- W1900817811 cites W2271710655 @default.
- W1900817811 cites W2289322704 @default.
- W1900817811 cites W2300654763 @default.
- W1900817811 cites W2336868589 @default.
- W1900817811 cites W2339329778 @default.
- W1900817811 cites W2397578269 @default.
- W1900817811 cites W2411812738 @default.
- W1900817811 cites W2413702326 @default.
- W1900817811 cites W2417703957 @default.
- W1900817811 cites W2418960547 @default.
- W1900817811 cites W2463045643 @default.
- W1900817811 cites W2474284528 @default.
- W1900817811 cites W263481497 @default.
- W1900817811 cites W3147480104 @default.
- W1900817811 cites W335336991 @default.
- W1900817811 cites W343740181 @default.
- W1900817811 cites W4231995437 @default.
- W1900817811 cites W4245868295 @default.
- W1900817811 cites W4247589583 @default.
- W1900817811 cites W4252030004 @default.
- W1900817811 cites W4320800996 @default.
- W1900817811 cites W69347198 @default.
- W1900817811 cites W93669950 @default.
- W1900817811 doi "https://doi.org/10.1002/stem.5530110503" @default.