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- W1900991908 abstract "Abstract A dynamic actomyosin cytoskeleton drives many morphogenetic events. Conventional nonmuscle myosin-II (myosin) is a key chemomechanical motor that drives contraction of the actin cytoskeleton. We have explored the regulation of myosin activity by performing genetic screens to identify gene products that collaborate with myosin during Drosophila morphogenesis. Specifically, we screened for second-site noncomplementors of a mutation in the zipper gene that encodes the nonmuscle myosin-II heavy chain. We determined that a single missense mutation in the zipperEbr allele gives rise to its sensitivity to second-site noncomplementation. We then identify the Rho signal transduction pathway as necessary for proper myosin function. First we show that a lethal P-element insertion interacts genetically with zipper. Subsequently we show that this second-site noncomplementing mutation disrupts the RhoGEF2 locus. Next, we show that two EMS-induced mutations, previously shown to interact genetically with zipperEbr, disrupt the RhoA locus. Further, we have identified their molecular lesions and determined that disruption of the carboxyl-terminal CaaX box gives rise to their mutant phenotype. Finally, we show that RhoA mutations themselves can be utilized in genetic screens. Biochemical and cell culture analyses suggest that Rho signal transduction regulates the activity of myosin. Our studies provide direct genetic proof of the biological relevance of regulation of myosin by Rho signal transduction in an intact metazoan." @default.
- W1900991908 created "2016-06-24" @default.
- W1900991908 creator A5027296712 @default.
- W1900991908 creator A5052150727 @default.
- W1900991908 creator A5067200001 @default.
- W1900991908 date "2000-07-01" @default.
- W1900991908 modified "2023-10-17" @default.
- W1900991908 title "Genetic Analysis Demonstrates a Direct Link Between Rho Signaling and Nonmuscle Myosin Function During Drosophila Morphogenesis" @default.
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- W1900991908 doi "https://doi.org/10.1093/genetics/155.3.1253" @default.
- W1900991908 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1461226" @default.
- W1900991908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10978309" @default.
- W1900991908 hasPublicationYear "2000" @default.
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