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• W1901002642 abstract "ObjectiveTo study the routine use of the E-test for susceptibility testing of penicillin-resistant Streptococcus pneumoniae.MethodsA multicenter study of penicillin-resistant S. pneumoniae (PRSP) was carried out in Brittany, France (10 general hospitals, and two university hospitals including a coordinating center). Each hospital detected PRSP by the oxacillin (5-μg) disk method and determined the MICs of penicillin G, amoxicillin and cefotaxime by the E-test under routine conditions. All the PRSP strains were collected in a coordinating center and the MICs were checked by the agar dilution method. The classifications obtained from the MICs determined by the E-test and by the reference method were compared.ResultsBetween 1 July 1993 and 30 June 1994, 128 PRSP strains were collected. Agreement within 1 log2 dilution was obtained for only 62% of strains with benzylpenicillin, 72.5% with amoxicillin and 76% with cefotaxime. These data are well below published values. In addition, 52% of the strains found to be penicillin-resistant by the reference technique were of intermediate resistance according to the E-test. There were major differences in the quality of the results obtained by the participating laboratories.ConclusionsThere are problems of standardization in the routine use of the E-test. Microbiologists should therefore take particular care when performing the test and when reading the results, and ensure that reference strains are included in the assay. To study the routine use of the E-test for susceptibility testing of penicillin-resistant Streptococcus pneumoniae. A multicenter study of penicillin-resistant S. pneumoniae (PRSP) was carried out in Brittany, France (10 general hospitals, and two university hospitals including a coordinating center). Each hospital detected PRSP by the oxacillin (5-μg) disk method and determined the MICs of penicillin G, amoxicillin and cefotaxime by the E-test under routine conditions. All the PRSP strains were collected in a coordinating center and the MICs were checked by the agar dilution method. The classifications obtained from the MICs determined by the E-test and by the reference method were compared. Between 1 July 1993 and 30 June 1994, 128 PRSP strains were collected. Agreement within 1 log2 dilution was obtained for only 62% of strains with benzylpenicillin, 72.5% with amoxicillin and 76% with cefotaxime. These data are well below published values. In addition, 52% of the strains found to be penicillin-resistant by the reference technique were of intermediate resistance according to the E-test. There were major differences in the quality of the results obtained by the participating laboratories. There are problems of standardization in the routine use of the E-test. Microbiologists should therefore take particular care when performing the test and when reading the results, and ensure that reference strains are included in the assay." @default.
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• W1901002642 date "1997-08-01" @default.
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• W1901002642 title "Evaluation of the E-test for routine testing of the susceptibility of Streptococcus pneumoniae to benzylpenicillin, amoxicillin and cefotaxime" @default.
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• W1901002642 doi "https://doi.org/10.1111/j.1469-0691.1997.tb00285.x" @default.
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