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- W1901316615 abstract "The citrate utilization determinant from transposon Tn3411 has been cloned and sequenced, and its polypeptide products have been characterized in minicell experiments. The nucleotide sequence was determined for a 2,047-base-pair BglII restriction endonuclease fragment that includes the citrate determinant. This region contains an open reading frame that would encode a 431-amino-acid very hydrophobic polypeptide and which is preceded by a reasonable ribosomal binding site. However, the single polypeptide found in minicell experiments had an apparent molecular weight of 35,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis." @default.
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- W1901316615 date "1985-12-01" @default.
- W1901316615 modified "2023-10-14" @default.
- W1901316615 title "Nucleotide sequence of the gene determining plasmid-mediated citrate utilization" @default.
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- W1901316615 doi "https://doi.org/10.1128/jb.164.3.977-982.1985" @default.
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