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- W190147009 abstract "The powerful delivery capabilities of viral vectors have made them a prime tool in gene delivery for both basic research and therapy applications. Viral delivery tools combine both the cloning vector and delivery mechanism and they can target a wide variety of cell types both in vitro and in vivo with high efficiency. The lentivirus, specifically, has become a favored gene therapy tool for its unique ability to transduce dividing as well as terminally differentiated, non-dividing cells and to generate stable gene expression. These features are especially critical when attempting to genetically modify stem cells, hematopoetic cells, neurons and various tissues in vivo . Here we describe procedures to generate high titer lentivirus and transduce hPSCs. We then detail a method for site-specific integration of lentiviral vectors." @default.
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- W190147009 date "2012-01-01" @default.
- W190147009 modified "2023-09-27" @default.
- W190147009 title "Lentiviral Vector Systems for Transgene Delivery" @default.
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- W190147009 doi "https://doi.org/10.1016/b978-0-12-385473-5.00030-8" @default.
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