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• W1901789852 abstract "Leaf rust, caused by Puccinia triticina (Pt), is an important disease of wheat worldwide. New leaf rust resistance loci from diverse germplasm including wild relatives of wheat are continuously being identified. Many leaf rust resistance genes produce similar infection types and therefore are difficult to combine in a single genotype using conventional techniques. Identification of molecular markers linked with resistance genes can facilitate pyramiding of two or more genes in new cultivars. Leaf rust resistance gene Lr28 was transferred from Aegilops speltoides to common wheat. A doubled haploid (DH) population (192 lines) was developed from the cross PBW343+Lr24+Lr28/Lang. The parent PBW+Lr24+Lr28 also carries Lr26 and the alternate parent wheat cultivar Lang carries Lr24. The entire DH population was tested against Lr24 virulent Pt pathotype 104-1,(2),3,(6),(7),11,13 and segregation at leaf rust resistance loci Lr26 and Lr28 was observed. These genes produce similar seedling responses against Lr24 virulent Pt pathotype, therefore the Lr26-linked marker, Iag95, was used to identify a set of 123 DH lines that segregated monogenically for Lr28. This selected set was used for molecular studies. Bulked segregant analysis was carried out using 38 markers (24 SSR, 13 STS derived from EST and a gene based marker) located on chromosome 4AL, seven markers differentiated resistant and susceptible bulks. These markers were mapped on 123 DH lines. An eSTS marker mag3092 co-segregated with Lr28 and amplified a 200bp product in PBW343+Lr24+Lr28 and 225bp in Lang. The Ae. speltoides translocated segment that carries Lr28 was flanked by markers barc343 and wmc219. Marker mag3092 produced either a 225bp product or a 210+225bp doublet, when DNA templates from a set of Australian, Indian and Pakistani cultivars known not to possess Lr28 were used. These results demonstrated the robustness of Lr28 linked marker mag3092 for marker assisted selection in diverse genetic backgrounds." @default.
• W1901789852 created "2016-06-24" @default.
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• W1901789852 date "2014-08-01" @default.
• W1901789852 modified "2023-09-26" @default.
• W1901789852 title "Identification of a co-dominant eSTS marker linked with leaf rust resistance gene 'Lr28' in wheat ('Triticum aestivum' L.)" @default.
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