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- W1902227489 abstract "Immobilization of proteins, nucleic acids, and other bioligands requires selection of the proper bioligand for the specific application. This decision influences all others: the matrix is chosen and activated by the method that is most appropriate for this specific application and the ligand is coupled under conditions dictated by the activation method and the nature of the ligand (e.g., is it a labile protein or a sturdy enzyme cofactor?). There are many matrices and activation and coupling methods, and new ones are constantly being developed. This unit provides three protocols for activating and coupling proteins and other nucleophilic ligands to beaded agarose gel: cyanogen bromide, p-nitrophenyl chloroformate, and tresyl chloride." @default.
- W1902227489 created "2016-06-24" @default.
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- W1902227489 date "1995-06-01" @default.
- W1902227489 modified "2023-10-13" @default.
- W1902227489 title "Affinity Purification of Natural Ligands" @default.
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- W1902227489 doi "https://doi.org/10.1002/0471140864.ps0903s00" @default.
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