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- W1902362526 abstract "Background & objectives: Making centrifuged deposit smears from sputum to detect acid-fastbacilli (AFB) is considered hazardous. We carried out this study to stain the centrifuged depositswith carbol-fuchsin in sputum containers and to decolourize and counterstain their smearsmade on glass slides.Methods: The centrifuged deposits of 180 sputum samples from pulmonary tuberculosis patientswere used for making smears (initial deposit smears) and staining by Ziehl-Neelsen (ZN)method for the detection of AFB. Each of the sputum deposit was then treated with one ml of1 per cent carbol-fuchsin and a smear made between 2 to 3 h was then decolourized andcounterstained by the same procedures followed in ZN method (2 h stained deposit smear). Thecoded initial deposit smears and the corresponding 2 h stained deposit smears were read by thesame readers and the results compared.Results: One hundred and fifty (70 positive and 80 negative) 2 h stained deposit smears werecompared with initial deposit smears and the difference was not statistically significant.Interpretation & conclusion: Centrifuged deposits of sputum in sputum containers can be stainedby carbol-fuchsin within 2-3 h and their smears made subsequently on glass slides can then bedecolourized and counterstained by the procedures followed in ZN method for detection of AFBby light microscopy." @default.
- W1902362526 created "2016-06-24" @default.
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- W1902362526 date "2006-06-01" @default.
- W1902362526 modified "2023-09-28" @default.
- W1902362526 title "A novel method of staining acid-fast bacilli in sputum containers." @default.
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