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- W1903321293 abstract "A new mini-ColE1 plasmid, designated pAC105, was isolated. It has a molecular weight of 1.6 X 10(6) and carries information for its self-replication as well as information for conferring colicin E1 immunity upon its host. Furthermore, pAC105 undergoes replication in the presence of chloramphenicol even when a foreign deoxyribonucleic acid (pSC101) is inserted into its single EcoRI restriction site. Studies in minicell-producing strains demonstrate that pAC105 codes for only two or three polypeptides of low molecular weight. The advantages of using it as a molecular cloning vehicle are discussed." @default.
- W1903321293 created "2016-06-24" @default.
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- W1903321293 date "1977-01-01" @default.
- W1903321293 modified "2023-09-22" @default.
- W1903321293 title "New mini-ColE1 as a molecular cloning vehicle" @default.
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- W1903321293 doi "https://doi.org/10.1128/jb.129.1.358-366.1977" @default.
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