Matches in SemOpenAlex for { <https://semopenalex.org/work/W1903485990> ?p ?o ?g. }
- W1903485990 endingPage "S15" @default.
- W1903485990 startingPage "S1" @default.
- W1903485990 abstract "Epithelial-mesenchymal transition (EMT) describes a process whereby polarized epithelial cells with restricted migration transform into elongated spindle-shaped mesenchymal cells with enhanced motility and invasiveness. Although there are some molecular markers for this process, including the down-regulation of E-cadherin, our understanding of plasma membrane (PM) and associated proteins involved in EMT is limited. To specifically explore molecular alterations occurring at the PM, we used the cationic colloidal silica isolation technique to purify PM fractions from epithelial Madin-Darby canine kidney cells during Ras/TGF-β-mediated EMT. Proteins in the isolated membrane fractions were separated by one-dimensional SDS-PAGE and subjected to nano-LC-MS/MS-based protein identification. In this study, the first membrane protein analysis of an EMT model, we identified 805 proteins and determined their differential expression using label-free spectral counting. These data reveal that Madin-Darby canine kidney cells switch from cadherin-mediated to integrin-mediated adhesion following Ras/TGF-β-mediated EMT. Thus, during the EMT process, E-cadherin, claudin 4, desmoplakin, desmoglein-2, and junctional adhesion molecule A were down-regulated, whereas integrins α6β1, α3β1, α2β1, α5β1, αVβ1, and αVβ3 along with their extracellular ligands collagens I and V and fibronectin had increased expression levels. Conspicuously, Wnt-5a expression was elevated in cells undergoing EMT, and transient Wnt-5a siRNA silencing attenuated both cell migration and invasion in these cells. Furthermore, Wnt-5a expression suppressed canonical Wnt signaling induced by Wnt-3a. Wnt-5a may act through the planar cell polarity pathway of the non-canonical Wnt signaling pathway as several of the components and modulators (Wnt-5a, -5b, frizzled 6, collagen triple helix repeat-containing protein 1, tyrosine-protein kinase 7, RhoA, Rac, and JNK) were found to be up-regulated during Ras/TGF-β-mediated EMT." @default.
- W1903485990 created "2016-06-24" @default.
- W1903485990 creator A5011373233 @default.
- W1903485990 creator A5012715816 @default.
- W1903485990 creator A5015382939 @default.
- W1903485990 creator A5036737482 @default.
- W1903485990 creator A5058750362 @default.
- W1903485990 creator A5061700388 @default.
- W1903485990 creator A5066701356 @default.
- W1903485990 date "2011-02-01" @default.
- W1903485990 modified "2023-10-17" @default.
- W1903485990 title "Proteomics Profiling of Madin-Darby Canine Kidney Plasma Membranes Reveals Wnt-5a Involvement during Oncogenic H-Ras/TGF-β-mediated Epithelial-Mesenchymal Transition" @default.
- W1903485990 cites W1488067750 @default.
- W1903485990 cites W1525035494 @default.
- W1903485990 cites W1565561072 @default.
- W1903485990 cites W1576773659 @default.
- W1903485990 cites W1744742580 @default.
- W1903485990 cites W1760982454 @default.
- W1903485990 cites W1770349940 @default.
- W1903485990 cites W1850975011 @default.
- W1903485990 cites W1966336179 @default.
- W1903485990 cites W1967854759 @default.
- W1903485990 cites W1968102802 @default.
- W1903485990 cites W1977657840 @default.
- W1903485990 cites W1979845971 @default.
- W1903485990 cites W1981593008 @default.
- W1903485990 cites W1985705162 @default.
- W1903485990 cites W1987473368 @default.
- W1903485990 cites W1990076643 @default.
- W1903485990 cites W1994115546 @default.
- W1903485990 cites W1996118868 @default.
- W1903485990 cites W2004886209 @default.
- W1903485990 cites W2007446347 @default.
- W1903485990 cites W2014090327 @default.
- W1903485990 cites W2020018374 @default.
- W1903485990 cites W2025506781 @default.
- W1903485990 cites W2025647419 @default.
- W1903485990 cites W2030257046 @default.
- W1903485990 cites W2039298921 @default.
- W1903485990 cites W2048591769 @default.
- W1903485990 cites W2049243908 @default.
- W1903485990 cites W2053497293 @default.
- W1903485990 cites W2054014973 @default.
- W1903485990 cites W2055097300 @default.
- W1903485990 cites W2061769845 @default.
- W1903485990 cites W2063011414 @default.
- W1903485990 cites W2063501462 @default.
- W1903485990 cites W2063548589 @default.
- W1903485990 cites W2064153835 @default.
- W1903485990 cites W2069440750 @default.
- W1903485990 cites W2069524101 @default.
- W1903485990 cites W2070058781 @default.
- W1903485990 cites W2073728422 @default.
- W1903485990 cites W2075093878 @default.
- W1903485990 cites W2076583198 @default.
- W1903485990 cites W2077462602 @default.
- W1903485990 cites W2079796219 @default.
- W1903485990 cites W2082151233 @default.
- W1903485990 cites W2088926937 @default.
- W1903485990 cites W2093048036 @default.
- W1903485990 cites W2097689792 @default.
- W1903485990 cites W2097829282 @default.
- W1903485990 cites W2099011309 @default.
- W1903485990 cites W2100592123 @default.
- W1903485990 cites W2101014673 @default.
- W1903485990 cites W2101244168 @default.
- W1903485990 cites W2108119086 @default.
- W1903485990 cites W2114286636 @default.
- W1903485990 cites W2116102471 @default.
- W1903485990 cites W2123998270 @default.
- W1903485990 cites W2132695443 @default.
- W1903485990 cites W2134749398 @default.
- W1903485990 cites W2137530154 @default.
- W1903485990 cites W2139458634 @default.
- W1903485990 cites W2140551998 @default.
- W1903485990 cites W2143810421 @default.
- W1903485990 cites W2147865927 @default.
- W1903485990 cites W2148632411 @default.
- W1903485990 cites W2148736421 @default.
- W1903485990 cites W2152770371 @default.
- W1903485990 cites W2152983466 @default.
- W1903485990 cites W2153550523 @default.
- W1903485990 cites W2154041910 @default.
- W1903485990 cites W2157055702 @default.
- W1903485990 cites W2159096438 @default.
- W1903485990 cites W2160110467 @default.
- W1903485990 cites W2160949062 @default.
- W1903485990 cites W2161260747 @default.
- W1903485990 cites W2165046771 @default.
- W1903485990 cites W2168796463 @default.
- W1903485990 cites W2171204703 @default.
- W1903485990 cites W2416595416 @default.
- W1903485990 cites W4231041617 @default.
- W1903485990 doi "https://doi.org/10.1074/mcp.m110.001131" @default.
- W1903485990 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3033669" @default.
- W1903485990 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20511395" @default.
- W1903485990 hasPublicationYear "2011" @default.
- W1903485990 type Work @default.