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• W1904043633 abstract "ABSTRACT Pieces of both unfixed and fixed rat adrenal cortex were frozen and cut with a cryoultramicrotome. In unstained frozen sections of unfixed adrenals patches of heterochromatin were seen in the nucleus, while numerous round uniformly dense structures, the mitochondria, could be identified in the cytoplasm. Lipid droplets appear as small light round areas. Staining the sections with potassium permanganate, phosphotungstic acid, or uranyl acetate, or immersing them in glutaraldehyde, increased the contrast of cell organelles. In frozen sections of fixed material, membranes became visible in negative contrast. Mitochondria display vesicular cristae, whereas the Golgi complex, lysosomes and peroxisomes are easily recognizable. The possibility of cutting frozen sections of unfixed material with reasonable morphological preservation allows the fine-structural localization of steroids in adrenal cells after applying autoradiographic emulsion to the sections." @default.
• W1904043633 created "2016-06-24" @default.
• W1904043633 creator A5018609442 @default.
• W1904043633 date "1977-10-01" @default.
• W1904043633 modified "2023-09-25" @default.
• W1904043633 title "Ultrastructure of the adrenal cortex in frozen thin sections" @default.
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• W1904043633 doi "https://doi.org/10.1242/jcs.27.1.303" @default.
• W1904043633 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/591579" @default.
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