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• W1904057306 abstract "Four regulated promoters that direct the transcription of genes (i.e., korA, tra, kilB, and korB) involved in the transfer of the Streptomyces plasmid pIJ101 were isolated following the in vitro fusion of plasmid DNA fragments to a promoterless gene encoding the S. lividans extracellular enzyme beta-galactosidase. Introduction of pIJ101 into cells carrying each of these promoter-lac fusions resulted in decreased lac expression. The sites of initiation of transcription by the promoter sequences were identified by primer extension experiments, and the DNA sequences specifically required for promoter activity and regulation by pIJ101-encoded functions were determined by deletion analysis. The data obtained indicate that the korB locus encodes a repressor that regulates its own transcription, as well as transcription of the kilB promoter; korA and tra are transcribed from overlapping divergent promoters that are coregulated by the korA gene product. Common DNA sequence domains within coregulated promoters allowed the identification of putative binding sites for each of the kor gene products." @default.
• W1904057306 created "2016-06-24" @default.
• W1904057306 creator A5006386713 @default.
• W1904057306 creator A5025538163 @default.
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• W1904057306 date "1989-11-01" @default.
• W1904057306 modified "2023-09-23" @default.
• W1904057306 title "Identification and analysis of transcriptional regulatory signals for the kil and kor loci of Streptomyces plasmid pIJ101" @default.
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• W1904057306 doi "https://doi.org/10.1128/jb.171.11.5768-5775.1989" @default.
• W1904057306 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/210435" @default.
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