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- W1905071397 abstract "ABSTRACT Motoneurons were identified in vitro by a new method using the SCI monoclonal antibody. They constituted up to 30 % of total neurons in cultures of whole spinal cord from 4.5-day chicken embryos, and survived for at least 5 days in the presence of muscle extract, but not in its absence. By contrast, other neurons and floor-plate cells survived without muscle-derived factors. Motoneurons were purified to homogeneity by ‘panning’ on dishes coated with SCI antibody; they developed rapidly even in the absence of other spinal cells. Concentrations of muscle extract required for half-maximal motoneuron survival were indistinguishable in pure and mixed cultures, suggesting that muscle-derived factors act directly on motoneurons. Other purified growth factors tested, including ciliary neurotrophic factor, did not have the survival-promoting activity of muscle." @default.
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- W1905071397 date "1991-01-01" @default.
- W1905071397 modified "2023-09-27" @default.
- W1905071397 title "Survival <i>in vitro</i> of motoneurons identified or purified by novel antibodybased methods is selectively enhanced by muscle-derived factors" @default.
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- W1905071397 doi "https://doi.org/10.1242/dev.111.1.221" @default.
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