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- W1905089013 abstract "In this study we design new fabrication techniques and demonstrate the potential of using dense CO2 (i.e. high pressure carbon dioxide that possess a liquid-like density) for facilitating crucial steps in the fabrication of plastic lab-on-a-chip (LOC) micro-devices by embedding biomolecules at temperatures well below the plastic’s glass transition temperature (Tg). The polymer polystyrene (PS) is the plastic that was used in this study and has a Tg of ~105C. The Tg is the temperature at which the plastic becomes rubbery and deformable, below this temperature it is glassy and acts as a brittle solid. These new techniques are environmentally friendly and done without the use of a clean room. Carbon dioxide at 40C and between 4.48 and 6.89 MPa was used to immobilize the biologically active molecule, β-galactosidase (β-gal), on the surface of PS micro-channels. To our knowledge, this is the first time dense CO2 has been used to directly immobilize an enzyme in a micro-channel. β-gal activity was maintained, and shown via a fluorescent reaction product, after enzyme immobilization and micro-channel capping by the designed fabrication steps at 40C and pressures up to 6.89 MPa. ____________________________________________________________________________ _*Department of Chemical and Biomolecular Engineering, 140 West 19th Ave, Columbus, OH 43210. The primary collaborators on this project were my advisors, Dr. David L. Tomasko at The Ohio State University and Dr. Fariba Dehghani of the University of Sydney, Sydney, Australia. This work has been published in Biomacromolecules. This project was funded by the National Science Foundation (EEC-0425626)." @default.
- W1905089013 created "2016-06-24" @default.
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- W1905089013 date "2008-04-01" @default.
- W1905089013 modified "2023-09-24" @default.
- W1905089013 title "A Novel Technique for Fabricating Plastic Lab-on-a-Chip Devices with an Immobilized Enzyme" @default.
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