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• W1906468684 abstract "In recent years, Anaplasma phagocytophilum and Rickettsia spp. have been detected in Ixodes ricinus in Germany and a focal distribution has been suggested for A. phagocytophilum. In the present study the prevalence of A. phagocytophilum and spotted fever group (SFG) rickettsiae was investigated in I. ricinus. DNA-extracts were taken from 2,862 unfed I. ricinus ticks (adults and nymphs) from eight sites in Munich, sampled over a five-month period. Single samples from three comparative sites outside of Munich were also included. A real-time PCR targeting the msp2 gene of A. phagocytophilum was used for screening and a nested PCR targeting the 16S rRNA gene for sequencing of 30% of positives. Screening for Rickettsia spp. was performed with a PCR targeting the citrate synthase gene (gltA), followed by PCRs detecting the ompA gene for all gltA positives, and the ompB and 16S rRNA genes for clarifying results of some samples. The overall prevalence was 2.90% (95% CI 2.27 to 3.48%) for A. phagocytophilum and 5.28% (95% CI 4.31 to 6.17%) for SFG rickettsiae. Only 0.31% of the ticks investigated were coinfected. Statistical analysis revealed that prevalence of A. phagocytophilum in ticks from city parks in Munich was significantly higher than in ticks from natural forest, whereas the prevalence of Rickettsia spp. was the opposite. For both, the prevalence in adults was significantly higher than in nymphs. Although wide ranges of prevalence were observed monthly, the variations were not significant along the observational period. Sequence analysis of 16S rRNA PCR products (n=31) revealed 100% homology to Ehrlichia sp. “Frankonia 2”, only one differed in one nucleotide position. All differed in one nucleotide position from the HGA agent detected in human patients. All rickettsial PCR products were also sequenced. All gltA sequences of R. helvetica (n=138) were 100% identical to each other and differed in one nucleotide position from the prototype sequence. Two different R. monacensis strains (n=13) were detected, which differed in up to 4 nucleotide positions in gltA, ompA and ompB. Further rickettsial strains (n=3) most probably belonging to rickettsial endosymbionts were also found. These results show, by molecular methods, a wide distribution of A. phagocytophilum and SFG rickettsiae in I. ricinus ticks in Southern Germany. SFG rickettsiae which are thought to be involved in human disease (R. helvetica and R. monacensis) had a significantly higher prevalence in natural forest areas. Prevalence of A. phagocytophilum was significantly higher in city parks; the genetic strain has not yet been associated with human infection." @default.
• W1906468684 created "2016-06-24" @default.
• W1906468684 creator A5025568235 @default.
• W1906468684 date "2008-07-18" @default.
• W1906468684 modified "2023-09-23" @default.
• W1906468684 title "Prevalence and genetic analysis of Anaplasma phagocytophilum and Spotted Fever Group rickettsiae in the tick Ixodes ricinus in urban and periurban sites in southern Germany" @default.
• W1906468684 cites W1486304048 @default.
• W1906468684 cites W150336576 @default.
• W1906468684 cites W1513601800 @default.
• W1906468684 cites W1522728938 @default.
• W1906468684 cites W1544010300 @default.
• W1906468684 cites W1560308669 @default.
• W1906468684 cites W1573676855 @default.
• W1906468684 cites W1575732614 @default.
• W1906468684 cites W1644430168 @default.
• W1906468684 cites W1749586573 @default.
• W1906468684 cites W1767962536 @default.
• W1906468684 cites W1846819366 @default.
• W1906468684 cites W1852073127 @default.
• W1906468684 cites W1915335956 @default.
• W1906468684 cites W1937368349 @default.
• W1906468684 cites W1956813439 @default.
• W1906468684 cites W1956842074 @default.
• W1906468684 cites W1964472937 @default.
• W1906468684 cites W1968017600 @default.
• W1906468684 cites W1972427522 @default.
• W1906468684 cites W1973039290 @default.
• W1906468684 cites W1978878912 @default.
• W1906468684 cites W1981454988 @default.
• W1906468684 cites W1982371107 @default.
• W1906468684 cites W1983620699 @default.
• W1906468684 cites W1986451416 @default.
• W1906468684 cites W1986630978 @default.
• W1906468684 cites W1988516778 @default.
• W1906468684 cites W1990330114 @default.
• W1906468684 cites W1992389768 @default.
• W1906468684 cites W1992869728 @default.
• W1906468684 cites W1995301267 @default.
• W1906468684 cites W1997167941 @default.
• W1906468684 cites W1999765497 @default.
• W1906468684 cites W2006000408 @default.
• W1906468684 cites W2006007901 @default.
• W1906468684 cites W2007756426 @default.
• W1906468684 cites W2007871007 @default.
• W1906468684 cites W2009389303 @default.
• W1906468684 cites W2010481094 @default.
• W1906468684 cites W2016518848 @default.
• W1906468684 cites W2018524890 @default.
• W1906468684 cites W2018540177 @default.
• W1906468684 cites W2022036834 @default.
• W1906468684 cites W2022512513 @default.
• W1906468684 cites W2024038311 @default.
• W1906468684 cites W2029411362 @default.
• W1906468684 cites W2029685080 @default.
• W1906468684 cites W2030136297 @default.
• W1906468684 cites W2033339460 @default.
• W1906468684 cites W2034514399 @default.
• W1906468684 cites W2036137439 @default.
• W1906468684 cites W2036415660 @default.
• W1906468684 cites W2042266439 @default.
• W1906468684 cites W2042989180 @default.
• W1906468684 cites W2044074704 @default.
• W1906468684 cites W2044476439 @default.
• W1906468684 cites W2045409905 @default.
• W1906468684 cites W2048113255 @default.
• W1906468684 cites W2048260840 @default.
• W1906468684 cites W2051089692 @default.
• W1906468684 cites W2053406737 @default.
• W1906468684 cites W2053875250 @default.
• W1906468684 cites W2057880980 @default.
• W1906468684 cites W2058927249 @default.
• W1906468684 cites W2059028211 @default.
• W1906468684 cites W2061808059 @default.
• W1906468684 cites W2061880687 @default.
• W1906468684 cites W2062040224 @default.
• W1906468684 cites W2064727223 @default.
• W1906468684 cites W2065769499 @default.
• W1906468684 cites W2067858365 @default.
• W1906468684 cites W2068088142 @default.
• W1906468684 cites W2070218428 @default.
• W1906468684 cites W2070750792 @default.
• W1906468684 cites W2073091325 @default.
• W1906468684 cites W2075774945 @default.
• W1906468684 cites W2084444788 @default.
• W1906468684 cites W2085068250 @default.
• W1906468684 cites W2086694559 @default.
• W1906468684 cites W2089112000 @default.
• W1906468684 cites W2089145757 @default.
• W1906468684 cites W2093548693 @default.
• W1906468684 cites W2094192168 @default.
• W1906468684 cites W2095675389 @default.
• W1906468684 cites W2097421005 @default.
• W1906468684 cites W2098072058 @default.
• W1906468684 cites W2100045150 @default.
• W1906468684 cites W2102343143 @default.
• W1906468684 cites W2102801292 @default.
• W1906468684 cites W2105371471 @default.
• W1906468684 cites W2105566905 @default.
• W1906468684 cites W2106225273 @default.
• W1906468684 cites W2107219351 @default.

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