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- W190694345 abstract "This chapter discusses carboxy-terminal protein sequence analysis using carboxypeptidase P (CPP) and electrospray mass spectrometry (ESMS). To test the applicability of the ESMS analysis method for the CPP digestion of proteins, recombinant E. coli derived human IL-3 was chosen as a model. IL-3 has a C-terminal sequence of …T-T-L-S-L-A-I-F-OH. The digestion conditions were used to digest 2.7 nmoles of IL-3. Aliquots of 500 pmoles were removed at several time points—10 min, 20 min, and 50 min—and analyzed by ESMS. At the 10 min time point, the appearance of multiply-charged ions representing consecutive losses of five C-terminal amino acids was observed in the mass spectrum. The average molecular weight of each truncated species can be determined through deconvolution of the mass spectrum. The chapter shows a closeup view of the (M+9H)+9 charge state of CPP-digested IL-3. The mass differences of these ions reflect the sequential loss of amino acids from full-length IL-3." @default.
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- W190694345 date "1993-01-01" @default.
- W190694345 modified "2023-10-18" @default.
- W190694345 title "Carboxy-terminal Protein Sequence Analysis using Carboxypeptidase P and Electrospray Mass Spectrometry" @default.
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- W190694345 doi "https://doi.org/10.1016/b978-0-12-058757-5.50055-x" @default.
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