FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1908631440> ?p ?o ?g. }

• W1908631440 endingPage "2983" @default.
• W1908631440 startingPage "2975" @default.
• W1908631440 abstract "Ca2+-induced exocytosis in neuronal and neuroendocrine cells involves ATP-dependent steps believed to `prime' vesicles for exocytosis. Primed, docked vesicles are released in response to Ca2+ influx through voltage-gated Ca2+ channels. Neutrophils, however, do not possess voltage-gated Ca2+ channels and appear to have no docked vesicles. Furthermore, neutrophils have several types of granules with markedly different Ca2+ requirements for exocytosis. These differential Ca2+ dependencies were used as a tool to investigate the ATP dependence of different granule populations. Here we demonstrate distinct ATP requirements for release of neutrophil granule populations, with respect to rate as well as amplitude. Intracellular ATP was depleted to various levels, and exocytosis was stimulated with different Ca2+concentrations and measured with the patch-clamp capacitance technique or by detecting enzyme release. Primary granule exocytosis displayed a distinct ATP dependence with an apparent Km of ∼80 μM ATP and no ATP-independent exocytosis. Release of secondary and tertiary granules displayed a more shallow ATP dependence (Km∼330 μM), and more than 50% of secondary and tertiary granules appeared not to need ATP at all for their release. Individual granules in human neutrophils have distinct ATP requirements for exocytosis, suggesting that the ATP-sensitive elements are localised to the granules. Primary granule exocytosis has a very low affinity for ATP. Furthermore, substantial ATP-independent exocytosis of secondary and tertiary granule occurs despite the absence of docked granules. These characteristics should help neutrophils to fulfil their bactericidal functions at poorly irrigated sites of infection with low glucose supply." @default.
• W1908631440 created "2016-06-24" @default.
• W1908631440 creator A5017278642 @default.
• W1908631440 creator A5023486091 @default.
• W1908631440 creator A5063814087 @default.
• W1908631440 date "2002-07-15" @default.
• W1908631440 modified "2023-09-27" @default.
• W1908631440 title "Granule-specific ATP requirements for Ca2+-induced exocytosis in human neutrophils. Evidence for substantial ATP-independent release" @default.
• W1908631440 cites W1508277854 @default.
• W1908631440 cites W1533838109 @default.
• W1908631440 cites W1561146956 @default.
• W1908631440 cites W1592007354 @default.
• W1908631440 cites W1965846572 @default.
• W1908631440 cites W1971016259 @default.
• W1908631440 cites W1979153662 @default.
• W1908631440 cites W1998413052 @default.
• W1908631440 cites W2004917770 @default.
• W1908631440 cites W2013910844 @default.
• W1908631440 cites W2016266703 @default.
• W1908631440 cites W2030067388 @default.
• W1908631440 cites W2032852796 @default.
• W1908631440 cites W2034044241 @default.
• W1908631440 cites W2045464110 @default.
• W1908631440 cites W2047211567 @default.
• W1908631440 cites W2056191227 @default.
• W1908631440 cites W2058595266 @default.
• W1908631440 cites W2058833411 @default.
• W1908631440 cites W2061978882 @default.
• W1908631440 cites W2086854917 @default.
• W1908631440 cites W2093486954 @default.
• W1908631440 cites W2101401045 @default.
• W1908631440 cites W2106997862 @default.
• W1908631440 cites W2112151664 @default.
• W1908631440 cites W2113728164 @default.
• W1908631440 cites W2138099953 @default.
• W1908631440 cites W2141260882 @default.
• W1908631440 cites W2157378804 @default.
• W1908631440 cites W2157840250 @default.
• W1908631440 cites W2234732939 @default.
• W1908631440 cites W2245141015 @default.
• W1908631440 cites W4205562860 @default.
• W1908631440 doi "https://doi.org/10.1242/jcs.115.14.2975" @default.
• W1908631440 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12082157" @default.
• W1908631440 hasPublicationYear "2002" @default.
• W1908631440 type Work @default.
• W1908631440 sameAs 1908631440 @default.
• W1908631440 citedByCount "16" @default.
• W1908631440 countsByYear W19086314402018 @default.
• W1908631440 countsByYear W19086314402019 @default.
• W1908631440 countsByYear W19086314402020 @default.
• W1908631440 countsByYear W19086314402021 @default.
• W1908631440 crossrefType "journal-article" @default.
• W1908631440 hasAuthorship W1908631440A5017278642 @default.
• W1908631440 hasAuthorship W1908631440A5023486091 @default.
• W1908631440 hasAuthorship W1908631440A5063814087 @default.
• W1908631440 hasConcept C12554922 @default.
• W1908631440 hasConcept C130316041 @default.
• W1908631440 hasConcept C151730666 @default.
• W1908631440 hasConcept C175969161 @default.
• W1908631440 hasConcept C41625074 @default.
• W1908631440 hasConcept C49039625 @default.
• W1908631440 hasConcept C49760210 @default.
• W1908631440 hasConcept C55493867 @default.
• W1908631440 hasConcept C79879829 @default.
• W1908631440 hasConcept C86803240 @default.
• W1908631440 hasConcept C95444343 @default.
• W1908631440 hasConceptScore W1908631440C12554922 @default.
• W1908631440 hasConceptScore W1908631440C130316041 @default.
• W1908631440 hasConceptScore W1908631440C151730666 @default.
• W1908631440 hasConceptScore W1908631440C175969161 @default.
• W1908631440 hasConceptScore W1908631440C41625074 @default.
• W1908631440 hasConceptScore W1908631440C49039625 @default.
• W1908631440 hasConceptScore W1908631440C49760210 @default.
• W1908631440 hasConceptScore W1908631440C55493867 @default.
• W1908631440 hasConceptScore W1908631440C79879829 @default.
• W1908631440 hasConceptScore W1908631440C86803240 @default.
• W1908631440 hasConceptScore W1908631440C95444343 @default.
• W1908631440 hasIssue "14" @default.
• W1908631440 hasLocation W19086314401 @default.
• W1908631440 hasLocation W19086314402 @default.
• W1908631440 hasOpenAccess W1908631440 @default.
• W1908631440 hasPrimaryLocation W19086314401 @default.
• W1908631440 hasRelatedWork W175220821 @default.
• W1908631440 hasRelatedWork W2008228882 @default.
• W1908631440 hasRelatedWork W2035664207 @default.
• W1908631440 hasRelatedWork W2048816285 @default.
• W1908631440 hasRelatedWork W2083133268 @default.
• W1908631440 hasRelatedWork W2086451945 @default.
• W1908631440 hasRelatedWork W2149707868 @default.
• W1908631440 hasRelatedWork W2283539280 @default.
• W1908631440 hasRelatedWork W4235264942 @default.
• W1908631440 hasRelatedWork W1586809413 @default.
• W1908631440 hasVolume "115" @default.
• W1908631440 isParatext "false" @default.
• W1908631440 isRetracted "false" @default.
• W1908631440 magId "1908631440" @default.
• W1908631440 workType "article" @default.