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- W1908797255 abstract "Coho salmon ( Oncorhynchus kisutch ) anti- Aeromonas salmonicida agglutinins were excluded from Sephadex G-200 and their activity was lost after partial reduction and alkylation followed by exposure to 2 M urea. Immunoelectrophoretic analysis of coho antiserum revealed anodic and cathodic populations of immunoglobulin (Ig), and the anti- A. salmonicida antibodies present 12 weeks after parenteral immunization were primarily anodic Ig. Antibodies were specifically purified by dissociation of A. salmonicida cell wall agglutinates with 15% NaCl. The purified Ig was a 17 S macroglobulin, and electron microscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that it was composed of four IgM-like subunits. The minimum coho antibody concentration required for visible agglutination of A. salmonicida cells was approximately 0.006 μg of Ig nitrogen/ml." @default.
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- W1908797255 date "1974-02-01" @default.
- W1908797255 modified "2023-09-23" @default.
- W1908797255 title "Characterization of Anti- Aeromonas salmonicida Antibodies from Coho Salmon" @default.
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- W1908797255 doi "https://doi.org/10.1128/iai.9.2.236-243.1974" @default.
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