FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1908816817> ?p ?o ?g. }

• W1908816817 endingPage "88" @default.
• W1908816817 startingPage "176" @default.
• W1908816817 abstract "Drug efflux pumps have been contributing factor(s) in the development of multidrug resistance in various clinically relevant bacteria. During efflux pump gene expression studies on mycobacteria, we have found a previously uncharacterized open reading frame (ORF) Rv2459 to be overexpressed in drug stressed conditions. The objective of the present study was to investigate the role of this ORF as a drug efflux pump, which might add new information in our understanding about the alternative mechanisms of drug resistance in mycobacteria.The open reading frame Rv2459 of Mycobacterium tuberculosis encoding a probable drug efflux protein has been cloned using pSD5 E.coli-Mycobacterium shuttle vector and overexpressed in M. tuberculosis H(37)Rv. This ORF was named as jefA. Overexpression of this gene in clones has been verified by real-time reverse transcription PCR. Minimum inhibitory concentrations (MICs) of recombinant as well as non-recombinant clones were determined by resazurin microtitre assay plate method (REMA) with and without efflux pump inhibitors carbonyl cyanide m-chlorophenylhydrazone (CCCP) and verapamil.In recombinant strains of M. tuberculosis, the overexpression of this gene led to an increase in MIC of anti-tubercular drugs isoniazid and ethambutol when tested by REMA. In the presence of CCCP and verapamil, the recombinant strains showed decrease in MIC for these drugs. Bioinformatic analysis has shown a close relation of JefA protein with drug efflux pumps of other clinically relevant bacteria. In homology derived structure prepared from nearest available model, it was observed that amino acids forming TMH 1, 8 and 11 participated in ethambutol specificity and those forming TMH 2, 7 and 10 participated in isoniazid specificity in JefA.The increased transcription of jefA leads to increased resistance to ethambutol and isoniazid in M. tuberculosis via efflux pump like mechanism and contributes in the development of resistance to these drugs. JefA amino acid sequence is well conserved among clinically important bacterial genera, which further provides evidence of being a potent drug efflux pump. The involvement in drug resistance and very little homology with any of the human proteins makes JefA important to be included in the list of potential drug targets." @default.
• W1908816817 created "2016-06-24" @default.
• W1908816817 creator A5019627093 @default.
• W1908816817 creator A5038184810 @default.
• W1908816817 creator A5057116586 @default.
• W1908816817 creator A5058780312 @default.
• W1908816817 creator A5068850222 @default.
• W1908816817 creator A5078490777 @default.
• W1908816817 creator A5081629840 @default.
• W1908816817 creator A5086687563 @default.
• W1908816817 date "2010-08-01" @default.
• W1908816817 modified "2023-09-23" @default.
• W1908816817 title "jefA (Rv2459), a drug efflux gene in Mycobacterium tuberculosis confers resistance to isoniazid & ethambutol." @default.
• W1908816817 cites W1499500424 @default.
• W1908816817 cites W1580619527 @default.
• W1908816817 cites W1914713595 @default.
• W1908816817 cites W1968064650 @default.
• W1908816817 cites W1973039152 @default.
• W1908816817 cites W1982168502 @default.
• W1908816817 cites W2001943039 @default.
• W1908816817 cites W2015642465 @default.
• W1908816817 cites W2028533496 @default.
• W1908816817 cites W2030839389 @default.
• W1908816817 cites W2032054300 @default.
• W1908816817 cites W2040105544 @default.
• W1908816817 cites W2041647495 @default.
• W1908816817 cites W2052644176 @default.
• W1908816817 cites W2055043387 @default.
• W1908816817 cites W205578041 @default.
• W1908816817 cites W2067784550 @default.
• W1908816817 cites W2081750628 @default.
• W1908816817 cites W2085662298 @default.
• W1908816817 cites W2100378493 @default.
• W1908816817 cites W2104258531 @default.
• W1908816817 cites W2106192857 @default.
• W1908816817 cites W2106882534 @default.
• W1908816817 cites W2107277218 @default.
• W1908816817 cites W2108325995 @default.
• W1908816817 cites W2109244971 @default.
• W1908816817 cites W2110593826 @default.
• W1908816817 cites W2112030533 @default.
• W1908816817 cites W2116128143 @default.
• W1908816817 cites W2118374299 @default.
• W1908816817 cites W2126585177 @default.
• W1908816817 cites W2127531034 @default.
• W1908816817 cites W2129026291 @default.
• W1908816817 cites W2129032245 @default.
• W1908816817 cites W2131256712 @default.
• W1908816817 cites W2139543498 @default.
• W1908816817 cites W2152075272 @default.
• W1908816817 cites W2156630451 @default.
• W1908816817 cites W2157744264 @default.
• W1908816817 cites W2338653879 @default.
• W1908816817 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20716818" @default.
• W1908816817 hasPublicationYear "2010" @default.
• W1908816817 type Work @default.
• W1908816817 sameAs 1908816817 @default.
• W1908816817 citedByCount "24" @default.
• W1908816817 countsByYear W19088168172012 @default.
• W1908816817 countsByYear W19088168172013 @default.
• W1908816817 countsByYear W19088168172014 @default.
• W1908816817 countsByYear W19088168172015 @default.
• W1908816817 countsByYear W19088168172016 @default.
• W1908816817 countsByYear W19088168172017 @default.
• W1908816817 countsByYear W19088168172018 @default.
• W1908816817 countsByYear W19088168172019 @default.
• W1908816817 countsByYear W19088168172021 @default.
• W1908816817 countsByYear W19088168172022 @default.
• W1908816817 countsByYear W19088168172023 @default.
• W1908816817 crossrefType "journal-article" @default.
• W1908816817 hasAuthorship W1908816817A5019627093 @default.
• W1908816817 hasAuthorship W1908816817A5038184810 @default.
• W1908816817 hasAuthorship W1908816817A5057116586 @default.
• W1908816817 hasAuthorship W1908816817A5058780312 @default.
• W1908816817 hasAuthorship W1908816817A5068850222 @default.
• W1908816817 hasAuthorship W1908816817A5078490777 @default.
• W1908816817 hasAuthorship W1908816817A5081629840 @default.
• W1908816817 hasAuthorship W1908816817A5086687563 @default.
• W1908816817 hasConcept C104317684 @default.
• W1908816817 hasConcept C114851261 @default.
• W1908816817 hasConcept C133936738 @default.
• W1908816817 hasConcept C142724271 @default.
• W1908816817 hasConcept C167625842 @default.
• W1908816817 hasConcept C200082930 @default.
• W1908816817 hasConcept C2776228421 @default.
• W1908816817 hasConcept C2776967927 @default.
• W1908816817 hasConcept C2777975735 @default.
• W1908816817 hasConcept C2778607973 @default.
• W1908816817 hasConcept C2781069245 @default.
• W1908816817 hasConcept C40767141 @default.
• W1908816817 hasConcept C47289529 @default.
• W1908816817 hasConcept C501593827 @default.
• W1908816817 hasConcept C55493867 @default.
• W1908816817 hasConcept C71924100 @default.
• W1908816817 hasConcept C86803240 @default.
• W1908816817 hasConcept C89423630 @default.
• W1908816817 hasConceptScore W1908816817C104317684 @default.
• W1908816817 hasConceptScore W1908816817C114851261 @default.

• next