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- W1909293860 abstract "The aim of the thesis was optimization of polymerase chain reaction for detection of genes encoding streptolysin S, streptococcal pyrogenic exotoxin G and streptococcal mitogenic exotoxin Z in group G streptococcal strains of human and animal origin. We introduced the polymerase chain reaction for detection of the gene encoding streptolysin S using primers sI and sII. The reaction mixture containing 2,75 mmol/l Mg2+ and the annealing temperature 53 °C gave detection limit of 28 pg. Further, the optimization of polymerase chain reaction for identification of the gene encoding streptococcal pyrogenic exotoxin G using primers gI and gII was performed. Under optimized PCR conditions (2,5 mmol/l Mg2+, annealing temperature 59 °C) the detection limit of this reaction was determined to be 300 pg. Furthermore, we optimized the polymerase chain reaction for detection of the gene encoding streptococcal mitogenic exotoxin Z using primers zI and zII. The reaction mixture containing 2,5 mmol/l Mg2+ and the annealing temperature 55 °C gave detection limit of 215 pg. Altogether 190 group G streptococcal strains of human and 67 strains of animal origin were investigated. Gene encoding streptolysin S was found in all studied strains. However, none of the strains harboured genes encoding streptococcal pyrogenic exotoxin G and streptococcal mitogenic exotoxin Z." @default.
- W1909293860 created "2016-06-24" @default.
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- W1909293860 date "2009-01-01" @default.
- W1909293860 modified "2023-09-26" @default.
- W1909293860 title "Průkaz genů kódujících vybrané faktory virulence u streptokoků se skupinovým antigenem G" @default.
- W1909293860 hasPublicationYear "2009" @default.
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