Matches in SemOpenAlex for { <https://semopenalex.org/work/W1909302889> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1909302889 endingPage "137" @default.
- W1909302889 startingPage "126" @default.
- W1909302889 abstract "IL-2-, IL-12-, and IFN-alpha-mediated signaling pathways were analyzed in primary NK cells and in the NK3.3 cell line. Gel mobility shift and immunoprecipitation analyses revealed that in addition to activating STAT3 (signal transducer and activator of transcription-3) and STAT5, IL-2 induced tyrosine and serine phosphorylation of STAT1 alpha, which formed IFN-gamma-activated sequence-binding complexes by itself and with STAT3. Although IL-2 and IFN-alpha activated STAT1 alpha and STAT5, IL-2 predominantly activated STAT5, while IFN-alpha predominantly activated STAT1 alpha. IL-2 induced less STAT1 alpha activation and IFN-alpha induced greater STAT5 activation in NK3.3 cells compared with preactivated primary NK cells. In NK3.3 cells, IL-2 induced comparable formation of c-fos promoter sis-inducible element IFN-gamma-activated sequence-binding complexes containing STAT3 alone with complexes containing STAT3 and STAT1 alpha, while in preactivated primary NK cells, it preferentially induced complexes containing STAT3 and STAT1 alpha. Thus, signaling in NK3.3 cells is not always identical with that in primary NK cells. In contrast to IL-2 and IFN-alpha, IL-12 induced strong tyrosine phosphorylation of STAT4 and variable weak phosphorylation of STAT3. However, supershift analyses using the c-fos promoter sis-inducible element probe showed that IL-12 activated STAT4, STAT1 alpha, and STAT3, and induced complexes containing STAT4 only, STAT4 with STAT1 alpha, STAT3 with STAT1 alpha, or STAT1 alpha only in preactivated primary NK cells. STAT1 alpha activation by IL-12 correlated with increased phosphorylation of serine, but not tyrosine. Finally, IL-2 induced tyrosine phosphorylation of JAK1 and JAK3, while IL-12 induced phosphorylation of JAK2 and TYK2 in both preactivated primary NK and NK3.3 cells. Differential phosphorylation and consequent differential activation of both separate and overlapping STAT proteins by IL-2, IL-12, and IFN-alpha may provide a molecular basis for the similarities and differences in the actions of these cytokines on NK cells." @default.
- W1909302889 created "2016-06-24" @default.
- W1909302889 creator A5034280359 @default.
- W1909302889 creator A5047297311 @default.
- W1909302889 creator A5055792486 @default.
- W1909302889 creator A5063483109 @default.
- W1909302889 creator A5080562741 @default.
- W1909302889 creator A5082754982 @default.
- W1909302889 date "1996-07-01" @default.
- W1909302889 modified "2023-10-14" @default.
- W1909302889 title "Differential utilization of Janus kinase-signal transducer activator of transcription signaling pathways in the stimulation of human natural killer cells by IL-2, IL-12, and IFN-alpha." @default.
- W1909302889 doi "https://doi.org/10.4049/jimmunol.157.1.126" @default.
- W1909302889 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8683106" @default.
- W1909302889 hasPublicationYear "1996" @default.
- W1909302889 type Work @default.
- W1909302889 sameAs 1909302889 @default.
- W1909302889 citedByCount "77" @default.
- W1909302889 countsByYear W19093028892013 @default.
- W1909302889 countsByYear W19093028892014 @default.
- W1909302889 countsByYear W19093028892015 @default.
- W1909302889 countsByYear W19093028892017 @default.
- W1909302889 countsByYear W19093028892018 @default.
- W1909302889 countsByYear W19093028892020 @default.
- W1909302889 countsByYear W19093028892022 @default.
- W1909302889 crossrefType "journal-article" @default.
- W1909302889 hasAuthorship W1909302889A5034280359 @default.
- W1909302889 hasAuthorship W1909302889A5047297311 @default.
- W1909302889 hasAuthorship W1909302889A5055792486 @default.
- W1909302889 hasAuthorship W1909302889A5063483109 @default.
- W1909302889 hasAuthorship W1909302889A5080562741 @default.
- W1909302889 hasAuthorship W1909302889A5082754982 @default.
- W1909302889 hasConcept C112392421 @default.
- W1909302889 hasConcept C11960822 @default.
- W1909302889 hasConcept C153911025 @default.
- W1909302889 hasConcept C171958077 @default.
- W1909302889 hasConcept C185592680 @default.
- W1909302889 hasConcept C2777003663 @default.
- W1909302889 hasConcept C2777553839 @default.
- W1909302889 hasConcept C2778277574 @default.
- W1909302889 hasConcept C2778923194 @default.
- W1909302889 hasConcept C2779391668 @default.
- W1909302889 hasConcept C32465701 @default.
- W1909302889 hasConcept C62478195 @default.
- W1909302889 hasConcept C65439459 @default.
- W1909302889 hasConcept C86803240 @default.
- W1909302889 hasConcept C95444343 @default.
- W1909302889 hasConceptScore W1909302889C112392421 @default.
- W1909302889 hasConceptScore W1909302889C11960822 @default.
- W1909302889 hasConceptScore W1909302889C153911025 @default.
- W1909302889 hasConceptScore W1909302889C171958077 @default.
- W1909302889 hasConceptScore W1909302889C185592680 @default.
- W1909302889 hasConceptScore W1909302889C2777003663 @default.
- W1909302889 hasConceptScore W1909302889C2777553839 @default.
- W1909302889 hasConceptScore W1909302889C2778277574 @default.
- W1909302889 hasConceptScore W1909302889C2778923194 @default.
- W1909302889 hasConceptScore W1909302889C2779391668 @default.
- W1909302889 hasConceptScore W1909302889C32465701 @default.
- W1909302889 hasConceptScore W1909302889C62478195 @default.
- W1909302889 hasConceptScore W1909302889C65439459 @default.
- W1909302889 hasConceptScore W1909302889C86803240 @default.
- W1909302889 hasConceptScore W1909302889C95444343 @default.
- W1909302889 hasIssue "1" @default.
- W1909302889 hasLocation W19093028891 @default.
- W1909302889 hasLocation W19093028892 @default.
- W1909302889 hasOpenAccess W1909302889 @default.
- W1909302889 hasPrimaryLocation W19093028891 @default.
- W1909302889 hasRelatedWork W1988362962 @default.
- W1909302889 hasRelatedWork W2008698342 @default.
- W1909302889 hasRelatedWork W2026999655 @default.
- W1909302889 hasRelatedWork W2065993983 @default.
- W1909302889 hasRelatedWork W2092932197 @default.
- W1909302889 hasRelatedWork W2323827802 @default.
- W1909302889 hasRelatedWork W2905092882 @default.
- W1909302889 hasRelatedWork W4230510698 @default.
- W1909302889 hasRelatedWork W4239327571 @default.
- W1909302889 hasRelatedWork W4244640182 @default.
- W1909302889 hasVolume "157" @default.
- W1909302889 isParatext "false" @default.
- W1909302889 isRetracted "false" @default.
- W1909302889 magId "1909302889" @default.
- W1909302889 workType "article" @default.