FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1909751364> ?p ?o ?g. }

• W1909751364 endingPage "4111" @default.
• W1909751364 startingPage "4104" @default.
• W1909751364 abstract "Abstract The mechanism of glucocorticoid-induced internucleosomal DNA cleavage and cytolysis of lymphatic cells is not known. Recent data (Compton, M.M., and Cidlowski, J.A. (1987) J. Biol. Chem. 262, 8288-8292) suggested that in vivo treatment of rat thymocytes with glucocorticoids induces a nucleolytic lysis gene product(s) responsible for lymphocytolysis. In this paper, the possibility that lymphocytolysis may result from glucocorticoid-induced nuclease(s) was examined. Using the rat thymocytes as a model system, we have shown by electrophoretic, enzymatic, and amino acid sequence analysis that the putative glucocorticoid-induced nucleases identified recently by Compton and Cidlowski are in fact H1, H1(0), and core histones, and their gross appearance is not the result of new histone protein synthesis, but a result of the release of histone-containing nucleosomes during chromatin breakdown. Evidence presented here shows that the putative induced nuclease activity is an artifact of the assay system employed. Because our data do not support induction of a glucocorticoid-induced nuclease(s), we examined the possibility that DNA cleavage might be induced by activation of a constitutive endogenous endonuclease. We have shown that it is possible to produce characteristic internucleosomal DNA cleavage of rat thymocytes, merely by incubating intact nuclei from untreated adrenalectomized rat thymocytes with Ca2+ and Mg2+ for a short period of time. However, in glucocorticoid-sensitive human CEM-C7 lymphocytes activation of internucleosomal DNA cleavage was independent of calcium uptake. We conclude that glucocorticoid induction of internucleosomal DNA fragmentation does not necessarily require expression of a new nuclease(s), but is the result of the activation of a constitutive endogenous endonuclease(s). Also, our data suggest that the mechanism which controls activation of internucleosomal DNA cleavage in rat thymocytes differs from that which operates in CEM-C7 lymphocytes." @default.
• W1909751364 created "2016-06-24" @default.
• W1909751364 creator A5062814155 @default.
• W1909751364 creator A5077708146 @default.
• W1909751364 date "1989-03-01" @default.
• W1909751364 modified "2023-09-26" @default.
• W1909751364 title "Glucocorticoid-induced Lymphocytolysis Is Not Mediated by an Induced Endonuclease" @default.
• W1909751364 cites W1495014693 @default.
• W1909751364 cites W1501288654 @default.
• W1909751364 cites W1514087839 @default.
• W1909751364 cites W1524424301 @default.
• W1909751364 cites W1541686953 @default.
• W1909751364 cites W1545130210 @default.
• W1909751364 cites W1550978696 @default.
• W1909751364 cites W1585446193 @default.
• W1909751364 cites W1589415602 @default.
• W1909751364 cites W1775749144 @default.
• W1909751364 cites W1981848770 @default.
• W1909751364 cites W1991946665 @default.
• W1909751364 cites W1994320352 @default.
• W1909751364 cites W2000136499 @default.
• W1909751364 cites W2004977301 @default.
• W1909751364 cites W2006416369 @default.
• W1909751364 cites W2014286993 @default.
• W1909751364 cites W2023893413 @default.
• W1909751364 cites W2035153986 @default.
• W1909751364 cites W2036562886 @default.
• W1909751364 cites W2059337254 @default.
• W1909751364 cites W2070628221 @default.
• W1909751364 cites W2072292948 @default.
• W1909751364 cites W2075507370 @default.
• W1909751364 cites W2081436336 @default.
• W1909751364 cites W2083379968 @default.
• W1909751364 cites W2084609653 @default.
• W1909751364 cites W2092315594 @default.
• W1909751364 cites W2096468699 @default.
• W1909751364 cites W2101462909 @default.
• W1909751364 cites W2104360623 @default.
• W1909751364 cites W2117828448 @default.
• W1909751364 cites W2170049808 @default.
• W1909751364 cites W22143531 @default.
• W1909751364 doi "https://doi.org/10.1016/s0021-9258(19)84968-2" @default.
• W1909751364 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2917990" @default.
• W1909751364 hasPublicationYear "1989" @default.
• W1909751364 type Work @default.
• W1909751364 sameAs 1909751364 @default.
• W1909751364 citedByCount "87" @default.
• W1909751364 countsByYear W19097513642018 @default.
• W1909751364 countsByYear W19097513642019 @default.
• W1909751364 crossrefType "journal-article" @default.
• W1909751364 hasAuthorship W1909751364A5062814155 @default.
• W1909751364 hasAuthorship W1909751364A5077708146 @default.
• W1909751364 hasBestOaLocation W19097513641 @default.
• W1909751364 hasConcept C134018914 @default.
• W1909751364 hasConcept C181199279 @default.
• W1909751364 hasConcept C185592680 @default.
• W1909751364 hasConcept C2777028655 @default.
• W1909751364 hasConcept C2780841215 @default.
• W1909751364 hasConcept C55493867 @default.
• W1909751364 hasConcept C86803240 @default.
• W1909751364 hasConcept C95444343 @default.
• W1909751364 hasConceptScore W1909751364C134018914 @default.
• W1909751364 hasConceptScore W1909751364C181199279 @default.
• W1909751364 hasConceptScore W1909751364C185592680 @default.
• W1909751364 hasConceptScore W1909751364C2777028655 @default.
• W1909751364 hasConceptScore W1909751364C2780841215 @default.
• W1909751364 hasConceptScore W1909751364C55493867 @default.
• W1909751364 hasConceptScore W1909751364C86803240 @default.
• W1909751364 hasConceptScore W1909751364C95444343 @default.
• W1909751364 hasIssue "7" @default.
• W1909751364 hasLocation W19097513641 @default.
• W1909751364 hasOpenAccess W1909751364 @default.
• W1909751364 hasPrimaryLocation W19097513641 @default.
• W1909751364 hasRelatedWork W1499152451 @default.
• W1909751364 hasRelatedWork W1592552075 @default.
• W1909751364 hasRelatedWork W1635821397 @default.
• W1909751364 hasRelatedWork W1974106448 @default.
• W1909751364 hasRelatedWork W1981348886 @default.
• W1909751364 hasRelatedWork W2041932686 @default.
• W1909751364 hasRelatedWork W2043721606 @default.
• W1909751364 hasRelatedWork W2066017722 @default.
• W1909751364 hasRelatedWork W2410470576 @default.
• W1909751364 hasRelatedWork W57272298 @default.
• W1909751364 hasVolume "264" @default.
• W1909751364 isParatext "false" @default.
• W1909751364 isRetracted "false" @default.
• W1909751364 magId "1909751364" @default.
• W1909751364 workType "article" @default.