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• W1909833334 endingPage "3111" @default.
• W1909833334 startingPage "3102" @default.
• W1909833334 abstract "The cis- and trans-acting components of the Rev regulatory pathway employed by equine infectious anemia virus (EIAV) to regulate and coordinate viral gene expression were examined in complementation experiments. Viral protein expression and mRNA expression were compared in cells transiently transfected with wild-type or mutant proviruses in combination with Rev expression plasmids. Mutation of the predicted rev gene abolished Gag protein synthesis, and this defect was complemented, in trans, by Rev. Analysis of viral mRNAs from transfected cells confirmed that EIAV expresses five major mRNAs: the full-length and singly spliced mRNAs contain introns and encode viral structural proteins while the three fully spliced mRNAs, encoding nonstructural genes, are generated by alternative splicing. Compared to cells transfected with the wild-type provirus, the intron-containing mRNAs produced from the rev-minus mutant were present at reduced levels in the nuclear RNA fraction and were not detected in the cytoplasm. This pattern of viral mRNA synthesis was restored to the wild-type pattern by providing Rev in trans. In contrast to the intron-containing mRNAs, cytoplasmic accumulation of the multiply spliced class of mRNAs was independent of Rev. Closer examination of the multiply spliced class of viral mRNAs by reverse transcriptase-PCR analysis revealed a Rev-dependent alternative splicing phenomenon. In the absence of Rev, proviruses expressed a four-exon mRNA at high levels; the addition of Rev caused both a decrease in the levels of the four-exon mRNA and the appearance of a related mRNA lacking exon 3. The cis-acting RNA elements that mediate Rev responsiveness were studied with deleted proviruses, which revealed that EIAV contains at least two elements located near the ends of envelope gene. Unlike the Rev-responsive elements in other retroviruses, the cis-acting regions of EIAV do not appear to form complex secondary structures." @default.
• W1909833334 created "2016-06-24" @default.
• W1909833334 creator A5028144588 @default.
• W1909833334 creator A5052997534 @default.
• W1909833334 creator A5060177702 @default.
• W1909833334 creator A5063851986 @default.
• W1909833334 date "1994-05-01" @default.
• W1909833334 modified "2023-10-13" @default.
• W1909833334 title "Equine infectious anemia virus trans-regulatory protein Rev controls viral mRNA stability, accumulation, and alternative splicing" @default.
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• W1909833334 doi "https://doi.org/10.1128/jvi.68.5.3102-3111.1994" @default.
• W1909833334 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/236800" @default.
• W1909833334 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8151775" @default.
• W1909833334 hasPublicationYear "1994" @default.
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