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• W1910367279 abstract "espanolEl objetivo de este estudio fue determinar la presencia de Helicobacter pylori en placa dental y en mucosa gastrica de pacientes adultos. Se seleccionaron 48 pacientes, que habian de someterse a endoscopia por indicacion medica. En todos los pacientes se recogio placa dental antes de la endoscopia. A partir de la placa dental se realizo extraccion de ADN, con finalidad de identificar H. pylori por el metodo de Reaccion en Cadena de la Polimerasa (RCP o PCR). Posteriormente, los pacientes fueron sometidos a endoscopia digestiva, mediante la cual se tomaron muestras de mucosa gastrica. A continuacion se extrajo ADN para la posterior identificacion de H . pylori por el metodo PCR. La presencia de H. pylori fue observada en el 100% de las muestras de placa dental y en el 66,67% de las de mucosa gastrica. De los 48 pacientes se constato que 42 (87,5%) presentaban alteraciones gastroduodenales y, entre ellos, 29 (69,05%) presentaron reaccion positiva para Helicobacter pylori en placa dental y en mucosa gastrica. Entre los 6 pacientes con endoscopia normal se encontraron 3 casos (50%) con presencia simultanea de H. pylori en placa dental y mucosa gastrica. Los resultados de este estudio sugieren la presencia simultanea de H. pylori en placa dental y mucosa gastrica en elevadas proporciones. Estos resultados permiten sugerir ademas que la placa dental puede constituir un reservorio importante de este microorganismo. EnglishThe objective of this study was to determine the prevalence of Helicobacter pylori in dental plaque and in gastric mucosa. 48 subjects that were submeted to endoscopy for medical indication were selected. In all the patients plaque samples were collected before the endoscopy. DNA was extracted from the samples and evaluated for the presence de H. pylori using Polymerase Chain Reaction(PCR) method. After collection of oral samples one biopsy was collected in biopsy gastric of each subject. DNA was extracted and H. pylori identified using PCR method. The presence of Helicobacter pylori was observed in 100% of the samples oral and in 66,67% of gastric mucosa. We verified that 42 pactients (87.5%) presented gastroduodenales alterations and 29 of them (69,05%) presented positive reaction for H. pylori in dental plaque and gastric mucosa. In the 6 subjects with normal endoscopy were registered 3 cases (50%) with simultaneous presence of H. pylori in dental plaque and in gastric mucosa. The results of this study suggest the simultaneous presence of H. pylori in dental plaque and gastric mucosa in high percentage and that the dental plaque can represent reservoirs for this pathogen. portuguesobjetivo deste estudo era determinar a presenca de Helicobacter Pylori na placa dental e na mucosa gastrica do pacientes adulto. 48 pacientes selecionaram-se, aquele la era ser posto sob a endoscopia pela indicacao medica. Todos os em pacientes a placa dental fez exame do abrigo antes da endoscopia. Do DNA dental da placa a extracao foi feita, com finalidade de identificar H. pylori pelo metodo da reacao cadeia do polymerasa (RCP ou PCR). Mais tarde, os pacientes foram postos sob a endoscopia digestiva, por meio de que amostras foram feitas exame da mucosa gastrica. O DNA seguinte para a identificacao mais atrasada de H foi extraido. Pylori por metodo PCR. A presenca de H. pylori foram observados nos 100% das as mostras da placa dental e dentro 66.67% da que les da mucosa gastrica. Dos 48 pacientes indicou-se que 42 (87.5%) presentaron alteracoes dos gastroduodenales e, entre eles, presentan reaccion positivo de 29 (69.05%) para Helicobacter pylori na placa dental e na mucosa gastrica. Entre os seis pacientes com endoscopia normal estavam tres casos (50%) com presenca simultânea de H. pylori na placa dental e mucousa gastrica. Os resultados deste estudo sugerem a presenca simultânea de H. pylori na placa dental e mucousa gastrica em proporcoes elevadas. Estes resultados reservam para sugerir alem que a lata dental da placa constitua o reservorio importante." @default.
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• W1910367279 date "2005-05-01" @default.
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• W1910367279 title "DETECCIÓN DE HELICOBACTER PYLORI EN PLACA DENTAL Y EN MUCOSA GÁSTRICA DE PACIENTES SOMETIDOS A ENDOSCOPIA DIGESTIVA." @default.
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