FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W191044924> ?p ?o ?g. }

• W191044924 endingPage "530" @default.
• W191044924 startingPage "526" @default.
• W191044924 abstract "OBJECTIVE: To explore the impact of IL-2- and IL-15-activated donor natural killer (NK) cell infusion on graft-versus-host-disease (GVHD) and graft-versus-leukemia (GVL) effect post allogeneic hematopoietic stem cell transplantation (allo-HSCT). METHODS: The C57BL/6 mice splenic NK cells were selected by microbeads, and then expanded in the media containing IL-2 and IL-15. The killing activity of NK cells was detected. In the leukemia mouse model, recipients (BALB/c) were intravenously inoculated with EL9611 leukemia cells 8 days before transplantation. Lethally irradiated BALB/c recipient mice were transplanted with 5 x 10(6) bone marrow cells (BMCs), or 5 x 10(6) BMCs plus 1 x 10(7) splenocytes with or without 1 x 10(7) activated NK cells. Additionally, NK cell infusion group mice were intraperitoneally injected with a mixture of IL-2 and IL-15 post transplant. Survival time, GVHD occurrence, lineage chimerism, TRBV spectra-typing were observed post transplant. RESULTS: The purity of isolated splenic NK cells was 95.7% - 97.1%. The killing activity of NK cells after activation was increased by 3 times. GVHD did not occurred in allogeneic BMCs infusion group, whereas did from 1 week after transplant in allogeneic BMCs + splenocytes infusion group. The severity of GVHD in total body irradiation (TBI) experimental group was significantly lower than in splenocytes infusion group (P < 0.05). The survival time was 9.5 - 14.0 d in TBI alone conditioning group. In leukemia mouse model, 100 day survival rate was 10% the rest of them were died of leukemia while in experimental group, the more than 100 days survival rate was 80% (P < 0.01). PB NK cells at 2 week post-transplant were 4.8% in experimental group and 2.8% in control group. NK cells recovery in experimental group was earlier than that in control group (P < 0.05). TRBV reconstitution was faster in experimental group than in control group, moreover, the number of TRBV family expression was more in experimental group than in control group which mainly expressed monoclone or oligo-clone. CONCLUSIONS: Donor alloreactive NK cells can be efficiently expanded and activated with IL-2 and IL-15. Donor activated NK cell infusion and IL-2, IL-15 treatment can promote immune reconstitution, mitigate GVHD and reduce leukemia relapse." @default.
• W191044924 created "2016-06-24" @default.
• W191044924 creator A5002714862 @default.
• W191044924 creator A5006739024 @default.
• W191044924 creator A5017868574 @default.
• W191044924 creator A5028204654 @default.
• W191044924 creator A5030108757 @default.
• W191044924 creator A5051043805 @default.
• W191044924 creator A5067241526 @default.
• W191044924 creator A5071044114 @default.
• W191044924 creator A5071443000 @default.
• W191044924 date "2008-08-01" @default.
• W191044924 modified "2023-09-25" @default.
• W191044924 title "[Experimental study on IL-2- and IL-15 application in allogeneic hematopoietic stem cell transplantation]." @default.
• W191044924 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19112915" @default.
• W191044924 hasPublicationYear "2008" @default.
• W191044924 type Work @default.
• W191044924 sameAs 191044924 @default.
• W191044924 citedByCount "1" @default.
• W191044924 countsByYear W1910449242019 @default.
• W191044924 crossrefType "journal-article" @default.
• W191044924 hasAuthorship W191044924A5002714862 @default.
• W191044924 hasAuthorship W191044924A5006739024 @default.
• W191044924 hasAuthorship W191044924A5017868574 @default.
• W191044924 hasAuthorship W191044924A5028204654 @default.
• W191044924 hasAuthorship W191044924A5030108757 @default.
• W191044924 hasAuthorship W191044924A5051043805 @default.
• W191044924 hasAuthorship W191044924A5067241526 @default.
• W191044924 hasAuthorship W191044924A5071044114 @default.
• W191044924 hasAuthorship W191044924A5071443000 @default.
• W191044924 hasConcept C109159458 @default.
• W191044924 hasConcept C126322002 @default.
• W191044924 hasConcept C189976730 @default.
• W191044924 hasConcept C203014093 @default.
• W191044924 hasConcept C2776694085 @default.
• W191044924 hasConcept C2776755627 @default.
• W191044924 hasConcept C2777408962 @default.
• W191044924 hasConcept C2778461978 @default.
• W191044924 hasConcept C2778880498 @default.
• W191044924 hasConcept C2779972918 @default.
• W191044924 hasConcept C2780007613 @default.
• W191044924 hasConcept C2780931953 @default.
• W191044924 hasConcept C28328180 @default.
• W191044924 hasConcept C2911091166 @default.
• W191044924 hasConcept C54355233 @default.
• W191044924 hasConcept C71924100 @default.
• W191044924 hasConcept C86803240 @default.
• W191044924 hasConceptScore W191044924C109159458 @default.
• W191044924 hasConceptScore W191044924C126322002 @default.
• W191044924 hasConceptScore W191044924C189976730 @default.
• W191044924 hasConceptScore W191044924C203014093 @default.
• W191044924 hasConceptScore W191044924C2776694085 @default.
• W191044924 hasConceptScore W191044924C2776755627 @default.
• W191044924 hasConceptScore W191044924C2777408962 @default.
• W191044924 hasConceptScore W191044924C2778461978 @default.
• W191044924 hasConceptScore W191044924C2778880498 @default.
• W191044924 hasConceptScore W191044924C2779972918 @default.
• W191044924 hasConceptScore W191044924C2780007613 @default.
• W191044924 hasConceptScore W191044924C2780931953 @default.
• W191044924 hasConceptScore W191044924C28328180 @default.
• W191044924 hasConceptScore W191044924C2911091166 @default.
• W191044924 hasConceptScore W191044924C54355233 @default.
• W191044924 hasConceptScore W191044924C71924100 @default.
• W191044924 hasConceptScore W191044924C86803240 @default.
• W191044924 hasIssue "8" @default.
• W191044924 hasLocation W1910449241 @default.
• W191044924 hasLocation W1910449242 @default.
• W191044924 hasOpenAccess W191044924 @default.
• W191044924 hasPrimaryLocation W1910449241 @default.
• W191044924 hasRelatedWork W1991602708 @default.
• W191044924 hasRelatedWork W2004151989 @default.
• W191044924 hasRelatedWork W2125686539 @default.
• W191044924 hasRelatedWork W2284988661 @default.
• W191044924 hasRelatedWork W2345269823 @default.
• W191044924 hasRelatedWork W2353866421 @default.
• W191044924 hasRelatedWork W2355422284 @default.
• W191044924 hasRelatedWork W2356489686 @default.
• W191044924 hasRelatedWork W2364058836 @default.
• W191044924 hasRelatedWork W2379978917 @default.
• W191044924 hasRelatedWork W2381429611 @default.
• W191044924 hasRelatedWork W2393711560 @default.
• W191044924 hasRelatedWork W2403814658 @default.
• W191044924 hasRelatedWork W2530503322 @default.
• W191044924 hasRelatedWork W2550378988 @default.
• W191044924 hasRelatedWork W2729736681 @default.
• W191044924 hasRelatedWork W2980593603 @default.
• W191044924 hasRelatedWork W3028802528 @default.
• W191044924 hasRelatedWork W3029068006 @default.
• W191044924 hasRelatedWork W3031048237 @default.
• W191044924 hasVolume "29" @default.
• W191044924 isParatext "false" @default.
• W191044924 isRetracted "false" @default.
• W191044924 magId "191044924" @default.
• W191044924 workType "article" @default.