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• W1911285871 endingPage "1056" @default.
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• W1911285871 abstract "The exact roles of disaccharidases and GLUT5 in the brush border membrane and GLUT2 in the basolateral membrane in the absorption of fructose across the intestine have not been fully determined. This paper describes characterization of fructose transport across the jejunal basolateral membrane using isolated membrane vesicles.Transport of fructose was measured using rapid filtration of vesicles. Luminal perfusion in vivo with glucose and fructose before vesicle preparation was used to assess modulation of GLUT2 activity. Western blotting measured the abundance of GLUT2 in the membrane.The maximal rate of transport for fructose was 1100 pmol/mg protein/s and the Michaelis constant was 16 mmol/L. Fructose and glucose could completely inhibit the transport of each other. Perfusion of the intestinal lumen with fructose or glucose saline for 4 hours produced a fourfold increase in maximal fructose transport.These data indicate that the one transport protein, GLUT2, is responsible for moving both fructose and glucose out of the enterocyte across the basolateral membrane under basal conditions. The activity of this, or a closely related carrier, is rapidly upregulated by the presence of hexoses in the intestinal lumen, explaining the potentiation of fructose absorption by luminal glucose and obviating any need to involve apical disaccharidases." @default.
• W1911285871 created "2016-06-24" @default.
• W1911285871 creator A5055327167 @default.
• W1911285871 date "1993-10-01" @default.
• W1911285871 modified "2023-10-10" @default.
• W1911285871 title "GLUT2 is the transporter for fructose across the rat intestinal basolateral membrane" @default.
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• W1911285871 doi "https://doi.org/10.1016/0016-5085(93)90948-c" @default.
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