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• W1911286823 abstract "Coevolving interactions between a plant population and its microbiota can potentially yield a rhizosphere enriched in metagenomes containing the blueprints for a vast array of natural products. We describe a method of isolating those metabolites through activity-based screening of soil metagenomic libraries. The method allows for the isolation of small molecules produced in vector–host expression systems containing large-insert DNA fragments extracted from the target plant rhizospheres. Allelopathic activities derived from selected clones were screened against a series of controls. Nonmetric multidimensional scaling (NMS) showed similar effects of the set of controls on lettuce growth, whereas annual bluegrass had a broader range of growth responses. Methanol extracts from clones indicating activity showed distinct patterns in grass seedling growth from the empty vector control, but the same extracts showed no effect on lettuce. The results indicate that the metagenomics method and bioassay screen of clone extracts are tools that can be used for initial determination of allelopathic activity from noncultured soil microbiota." @default.
• W1911286823 created "2016-06-24" @default.
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• W1911286823 date "2016-03-01" @default.
• W1911286823 modified "2023-10-11" @default.
• W1911286823 title "Uncovering Plant Growth-Mediating Allelochemicals Produced by Soil Microorganisms" @default.
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• W1911286823 doi "https://doi.org/10.1614/ws-d-15-00095.1" @default.
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