Matches in SemOpenAlex for { <https://semopenalex.org/work/W191144914> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W191144914 endingPage "150" @default.
- W191144914 startingPage "143" @default.
- W191144914 abstract "Considerable amounts (200 units/ml) of neuraminidase activity were detected in middle ear effusion of children (age 1 month-10 years) and its presence was highly correlated with the presence of Streptococcus pneumoniae. When isolates of this organism are cultured, neuraminidase activity appears in the growth medium during the exponential phase of growth. In order to study the role of this enzyme in the pathology of otitis media we have developed a method for its purification. The enzyme was purified over 5,800-fold by removing the organism and passing the culture broth through a series of affinity and ion-exchange columns. The overall yield was 2 mg enzyme protein and the final specific activity was 1.8 X 10(6) units/mg protein. A molecular weight of 65,000 was estimated by SDS-PAGE and gel filtration chromatography. The Stokes radius of neuraminidase was calculated to be 32 A, its isoelectric point was 7.2, and its pH optimum was 6.0. In terms of specificity, the enzyme catalyzed the hydrolysis of sialic acid linkages in mucin, glycoproteins, and gangliosides: bovine submaxillary mucin supported the highest catalytic efficiency, and alpha-1-antitrypsin the lowest. Neuraminidase acted on at least three linkage classes of substrates, alpha-2,6 and alpha-2,3 linkages of N-acetylneuraminic acid to galactose, and alpha-2,6 linkages to N-acetyl-galactosamine." @default.
- W191144914 created "2016-06-24" @default.
- W191144914 creator A5017165433 @default.
- W191144914 creator A5050349707 @default.
- W191144914 creator A5051421325 @default.
- W191144914 date "1989-01-01" @default.
- W191144914 modified "2023-09-24" @default.
- W191144914 title "Purification and Properties of Streptococcus pneumoniae Neuraminidase" @default.
- W191144914 doi "https://doi.org/10.1159/000469069" @default.
- W191144914 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2542012" @default.
- W191144914 hasPublicationYear "1989" @default.
- W191144914 type Work @default.
- W191144914 sameAs 191144914 @default.
- W191144914 citedByCount "37" @default.
- W191144914 countsByYear W1911449142012 @default.
- W191144914 countsByYear W1911449142013 @default.
- W191144914 countsByYear W1911449142014 @default.
- W191144914 countsByYear W1911449142015 @default.
- W191144914 countsByYear W1911449142017 @default.
- W191144914 countsByYear W1911449142018 @default.
- W191144914 countsByYear W1911449142023 @default.
- W191144914 crossrefType "journal-article" @default.
- W191144914 hasAuthorship W191144914A5017165433 @default.
- W191144914 hasAuthorship W191144914A5050349707 @default.
- W191144914 hasAuthorship W191144914A5051421325 @default.
- W191144914 hasConcept C108625454 @default.
- W191144914 hasConcept C134164806 @default.
- W191144914 hasConcept C179264091 @default.
- W191144914 hasConcept C181199279 @default.
- W191144914 hasConcept C185592680 @default.
- W191144914 hasConcept C193363816 @default.
- W191144914 hasConcept C198684833 @default.
- W191144914 hasConcept C2780115458 @default.
- W191144914 hasConcept C2781253189 @default.
- W191144914 hasConcept C43617362 @default.
- W191144914 hasConcept C501593827 @default.
- W191144914 hasConcept C50476208 @default.
- W191144914 hasConcept C55493867 @default.
- W191144914 hasConcept C86803240 @default.
- W191144914 hasConcept C89423630 @default.
- W191144914 hasConcept C96294017 @default.
- W191144914 hasConceptScore W191144914C108625454 @default.
- W191144914 hasConceptScore W191144914C134164806 @default.
- W191144914 hasConceptScore W191144914C179264091 @default.
- W191144914 hasConceptScore W191144914C181199279 @default.
- W191144914 hasConceptScore W191144914C185592680 @default.
- W191144914 hasConceptScore W191144914C193363816 @default.
- W191144914 hasConceptScore W191144914C198684833 @default.
- W191144914 hasConceptScore W191144914C2780115458 @default.
- W191144914 hasConceptScore W191144914C2781253189 @default.
- W191144914 hasConceptScore W191144914C43617362 @default.
- W191144914 hasConceptScore W191144914C501593827 @default.
- W191144914 hasConceptScore W191144914C50476208 @default.
- W191144914 hasConceptScore W191144914C55493867 @default.
- W191144914 hasConceptScore W191144914C86803240 @default.
- W191144914 hasConceptScore W191144914C89423630 @default.
- W191144914 hasConceptScore W191144914C96294017 @default.
- W191144914 hasIssue "3" @default.
- W191144914 hasLocation W1911449141 @default.
- W191144914 hasLocation W1911449142 @default.
- W191144914 hasOpenAccess W191144914 @default.
- W191144914 hasPrimaryLocation W1911449141 @default.
- W191144914 hasRelatedWork W1597296965 @default.
- W191144914 hasRelatedWork W1986902606 @default.
- W191144914 hasRelatedWork W1987262605 @default.
- W191144914 hasRelatedWork W1991238935 @default.
- W191144914 hasRelatedWork W2058555457 @default.
- W191144914 hasRelatedWork W2070548599 @default.
- W191144914 hasRelatedWork W2076341684 @default.
- W191144914 hasRelatedWork W2145515466 @default.
- W191144914 hasRelatedWork W2312609219 @default.
- W191144914 hasRelatedWork W2038586364 @default.
- W191144914 hasVolume "41" @default.
- W191144914 isParatext "false" @default.
- W191144914 isRetracted "false" @default.
- W191144914 magId "191144914" @default.
- W191144914 workType "article" @default.