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• W1911515883 endingPage "4067" @default.
• W1911515883 startingPage "4061" @default.
• W1911515883 abstract "Pseudomonas aeruginosa causes a chronic infection in the lungs of individuals with cystic fibrosis. The P. aeruginosa isolates from these infections, when grown under laboratory conditions, characteristically are mucoid and produce low levels of the more destructive virulence factors, such as exotoxin A and the proteases. We wanted to determine if during the chronic lung infections associated with CF, the expression of alginate was inversely correlated to the expression of exotoxin A, elastase, and the LasA protease. We measured the transcript accumulation of algD, a marker of alginate, toxA, the structural gene for exotoxin A, lasB, the structural gene for elastase, and lasA, the structural gene for LasA protease, from the sputum bacterial populations of 23 patients. In the 131 samples tested, we frequently detected transcripts from the four genes. When a Spearman rank correlation analysis was done on the samples, we found no correlation between algD transcript accumulation and toxA transcript accumulation. This result suggested that toxA was regulated independently of algD. Curiously, we found a positive correlation between algD transcript accumulation and both lasB and lasA transcript accumulation levels. This correlation may not indicate a direct association between algD and either lasA or lasB. More likely, it indicates a common regulatory element in a cascade of regulators or a common environmental cue that triggers transcription." @default.
• W1911515883 created "2016-06-24" @default.
• W1911515883 creator A5006555289 @default.
• W1911515883 creator A5033823459 @default.
• W1911515883 creator A5040072454 @default.
• W1911515883 creator A5086488324 @default.
• W1911515883 date "1997-10-01" @default.
• W1911515883 modified "2023-10-17" @default.
• W1911515883 title "Positive correlation of algD transcription to lasB and lasA transcription by populations of Pseudomonas aeruginosa in the lungs of patients with cystic fibrosis" @default.
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• W1911515883 doi "https://doi.org/10.1128/iai.65.10.4061-4067.1997" @default.
• W1911515883 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/175584" @default.
• W1911515883 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9317008" @default.
• W1911515883 hasPublicationYear "1997" @default.
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