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- W1912076240 endingPage "331" @default.
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- W1912076240 abstract "Expansion of the genetic alphabet by an unnatural base pair system provides a platform for the site-specific, enzymatic incorporation of extra, functional components into nucleic acids. Recently, several unnatural base pairs that exhibit high fidelity and efficiency in PCR have been developed. Functional groups of interest, such as fluorescent dyes, can be linked to the unnatural bases, and the modified base substrates are site-specifically incorporated into nucleic acids by polymerases. Furthermore, unique unnatural base pairs between fluorophore and quencher base analogs have been developed for imaging PCR amplification and as molecular beacons. Here, we describe the recent progress in the development of unnatural base pairs that function in PCR amplification and their applications as sensing and diagnostic tools." @default.
- W1912076240 created "2016-06-24" @default.
- W1912076240 creator A5004504880 @default.
- W1912076240 creator A5042765353 @default.
- W1912076240 creator A5066940547 @default.
- W1912076240 date "2011-04-01" @default.
- W1912076240 modified "2023-09-24" @default.
- W1912076240 title "Unnatural base pair systems for sensing and diagnostic applications" @default.
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- W1912076240 doi "https://doi.org/10.1586/erm.11.5" @default.
- W1912076240 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21463241" @default.
- W1912076240 hasPublicationYear "2011" @default.
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