Matches in SemOpenAlex for { <https://semopenalex.org/work/W1913308306> ?p ?o ?g. }
- W1913308306 endingPage "564" @default.
- W1913308306 startingPage "555" @default.
- W1913308306 abstract "Rapid neurotransmitter release depends on the Ca2+ sensor Synaptotagmin-1 (Syt1) and the SNARE complex formed by synaptobrevin, syntaxin-1 and SNAP-25. How Syt1 triggers release has been unclear, partly because elucidating high-resolution structures of Syt1-SNARE complexes has been challenging. An NMR approach based on lanthanide-induced pseudocontact shifts now reveals a dynamic binding mode in which basic residues in the concave side of the Syt1 C2B-domain β-sandwich interact with a polyacidic region of the SNARE complex formed by syntaxin-1 and SNAP-25. The physiological relevance of this dynamic structural model is supported by mutations in basic residues of Syt1 that markedly impair SNARE-complex binding in vitro and Syt1 function in neurons. Mutations with milder effects on binding have correspondingly milder effects on Syt1 function. Our results support a model whereby dynamic interaction facilitates cooperation between Syt1 and the SNAREs in inducing membrane fusion." @default.
- W1913308306 created "2016-06-24" @default.
- W1913308306 creator A5019294596 @default.
- W1913308306 creator A5037644100 @default.
- W1913308306 creator A5038452125 @default.
- W1913308306 creator A5039188699 @default.
- W1913308306 creator A5039376780 @default.
- W1913308306 creator A5044583992 @default.
- W1913308306 creator A5045511261 @default.
- W1913308306 creator A5050804320 @default.
- W1913308306 creator A5050902155 @default.
- W1913308306 creator A5053069183 @default.
- W1913308306 creator A5061751217 @default.
- W1913308306 creator A5061921730 @default.
- W1913308306 creator A5064072429 @default.
- W1913308306 creator A5065106268 @default.
- W1913308306 creator A5066688910 @default.
- W1913308306 creator A5067405307 @default.
- W1913308306 creator A5079500303 @default.
- W1913308306 creator A5081800735 @default.
- W1913308306 creator A5086543406 @default.
- W1913308306 creator A5089814380 @default.
- W1913308306 date "2015-06-01" @default.
- W1913308306 modified "2023-10-14" @default.
- W1913308306 title "Dynamic binding mode of a Synaptotagmin-1–SNARE complex in solution" @default.
- W1913308306 cites W1796620477 @default.
- W1913308306 cites W1822252127 @default.
- W1913308306 cites W1876590017 @default.
- W1913308306 cites W1916164999 @default.
- W1913308306 cites W1964375147 @default.
- W1913308306 cites W1965659132 @default.
- W1913308306 cites W1967409010 @default.
- W1913308306 cites W1969421890 @default.
- W1913308306 cites W1970930136 @default.
- W1913308306 cites W1971432065 @default.
- W1913308306 cites W1972171404 @default.
- W1913308306 cites W1976479871 @default.
- W1913308306 cites W1977903392 @default.
- W1913308306 cites W1980682773 @default.
- W1913308306 cites W1980740412 @default.
- W1913308306 cites W1981726470 @default.
- W1913308306 cites W1985321331 @default.
- W1913308306 cites W1991546697 @default.
- W1913308306 cites W1999062038 @default.
- W1913308306 cites W1999213641 @default.
- W1913308306 cites W2000003653 @default.
- W1913308306 cites W2002701489 @default.
- W1913308306 cites W2004827874 @default.
- W1913308306 cites W2009639277 @default.
- W1913308306 cites W2011516085 @default.
- W1913308306 cites W2011940688 @default.
- W1913308306 cites W2012434595 @default.
- W1913308306 cites W2016812630 @default.
- W1913308306 cites W2017759753 @default.
- W1913308306 cites W2018757497 @default.
- W1913308306 cites W2020593055 @default.
- W1913308306 cites W2021285837 @default.
- W1913308306 cites W2021331954 @default.
- W1913308306 cites W2027399226 @default.
- W1913308306 cites W2034403132 @default.
- W1913308306 cites W2037702696 @default.
- W1913308306 cites W2040224852 @default.
- W1913308306 cites W2052999464 @default.
- W1913308306 cites W2056293200 @default.
- W1913308306 cites W2058035029 @default.
- W1913308306 cites W2063253418 @default.
- W1913308306 cites W2067463218 @default.
- W1913308306 cites W2073850595 @default.
- W1913308306 cites W2075174077 @default.
- W1913308306 cites W2075402444 @default.
- W1913308306 cites W2076881120 @default.
- W1913308306 cites W2078230520 @default.
- W1913308306 cites W2079405491 @default.
- W1913308306 cites W2082176639 @default.
- W1913308306 cites W2086036400 @default.
- W1913308306 cites W2094337062 @default.
- W1913308306 cites W2101097180 @default.
- W1913308306 cites W2105537524 @default.
- W1913308306 cites W2107572237 @default.
- W1913308306 cites W2113029770 @default.
- W1913308306 cites W2115743000 @default.
- W1913308306 cites W2123355036 @default.
- W1913308306 cites W2125095292 @default.
- W1913308306 cites W2134371594 @default.
- W1913308306 cites W2139420483 @default.
- W1913308306 cites W2141518284 @default.
- W1913308306 cites W2143830427 @default.
- W1913308306 cites W2150670276 @default.
- W1913308306 cites W2159529647 @default.
- W1913308306 cites W2169142054 @default.
- W1913308306 cites W2169821755 @default.
- W1913308306 cites W2171279562 @default.
- W1913308306 cites W2313905315 @default.
- W1913308306 cites W2320400318 @default.
- W1913308306 cites W2326704249 @default.
- W1913308306 cites W4318442947 @default.
- W1913308306 doi "https://doi.org/10.1038/nsmb.3035" @default.
- W1913308306 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4496268" @default.