FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1913576888> ?p ?o ?g. }

• W1913576888 endingPage "C424" @default.
• W1913576888 startingPage "C415" @default.
• W1913576888 abstract "Recent studies have demonstrated that functionally discrete pools of phosphodiesterase (PDE) activity regulate distinct cellular functions. While the importance of localized pools of enzyme activity has become apparent, few studies have estimated enzyme activity within discrete subcellular compartments. Here we present an approach to estimate near-membrane PDE activity. First, total PDE activity is measured using traditional PDE activity assays. Second, known cAMP concentrations are dialyzed into single cells and the spatial spread of cAMP is monitored using cyclic nucleotide-gated channels. Third, mathematical models are used to estimate the spatial distribution of PDE activity within cells. Using this three-tiered approach, we observed two pharmacologically distinct pools of PDE activity, a rolipram-sensitive pool and an 8-methoxymethyl IBMX (8MM-IBMX)-sensitive pool. We observed that the rolipram-sensitive PDE (PDE4) was primarily responsible for cAMP hydrolysis near the plasma membrane. Finally, we observed that PDE4 was capable of blunting cAMP levels near the plasma membrane even when 100 μM cAMP were introduced into the cell via a patch pipette. Two compartment models predict that PDE activity near the plasma membrane, near cyclic nucleotide-gated channels, was significantly lower than total cellular PDE activity and that a slow spatial spread of cAMP allowed PDE activity to effectively hydrolyze near-membrane cAMP. These results imply that cAMP levels near the plasma membrane are distinct from those in other subcellular compartments; PDE activity is not uniform within cells; and localized pools of AC and PDE activities are responsible for controlling cAMP levels within distinct subcellular compartments." @default.
• W1913576888 created "2016-06-24" @default.
• W1913576888 creator A5032098701 @default.
• W1913576888 creator A5048563132 @default.
• W1913576888 creator A5051653179 @default.
• W1913576888 creator A5062264366 @default.
• W1913576888 creator A5064973035 @default.
• W1913576888 creator A5076118562 @default.
• W1913576888 creator A5077833017 @default.
• W1913576888 creator A5084394132 @default.
• W1913576888 date "2015-09-15" @default.
• W1913576888 modified "2023-09-23" @default.
• W1913576888 title "Estimating the magnitude of near-membrane PDE4 activity in living cells" @default.
• W1913576888 cites W1516500578 @default.
• W1913576888 cites W1554102421 @default.
• W1913576888 cites W1603793690 @default.
• W1913576888 cites W1823881256 @default.
• W1913576888 cites W1963636942 @default.
• W1913576888 cites W1965278565 @default.
• W1913576888 cites W1966389585 @default.
• W1913576888 cites W1972180454 @default.
• W1913576888 cites W1977109399 @default.
• W1913576888 cites W1979525072 @default.
• W1913576888 cites W1980580132 @default.
• W1913576888 cites W1987749939 @default.
• W1913576888 cites W1992860067 @default.
• W1913576888 cites W2003143337 @default.
• W1913576888 cites W2003646327 @default.
• W1913576888 cites W2004403903 @default.
• W1913576888 cites W2006036490 @default.
• W1913576888 cites W2017530576 @default.
• W1913576888 cites W2023915716 @default.
• W1913576888 cites W2024247640 @default.
• W1913576888 cites W2035926062 @default.
• W1913576888 cites W2045562023 @default.
• W1913576888 cites W2049514067 @default.
• W1913576888 cites W2063557162 @default.
• W1913576888 cites W2068320316 @default.
• W1913576888 cites W2074158980 @default.
• W1913576888 cites W2078403132 @default.
• W1913576888 cites W2097898429 @default.
• W1913576888 cites W2104479866 @default.
• W1913576888 cites W2112867165 @default.
• W1913576888 cites W2113094198 @default.
• W1913576888 cites W2120904086 @default.
• W1913576888 cites W2121200653 @default.
• W1913576888 cites W2122833172 @default.
• W1913576888 cites W2126666532 @default.
• W1913576888 cites W2133648715 @default.
• W1913576888 cites W2133896448 @default.
• W1913576888 cites W2134972637 @default.
• W1913576888 cites W2137599759 @default.
• W1913576888 cites W2145957163 @default.
• W1913576888 cites W2146048430 @default.
• W1913576888 cites W2147225829 @default.
• W1913576888 cites W2155427397 @default.
• W1913576888 cites W2158713438 @default.
• W1913576888 cites W2161421667 @default.
• W1913576888 cites W2166389885 @default.
• W1913576888 cites W2357552823 @default.
• W1913576888 cites W2417263156 @default.
• W1913576888 cites W4290366835 @default.
• W1913576888 cites W4301381893 @default.
• W1913576888 doi "https://doi.org/10.1152/ajpcell.00090.2015" @default.
• W1913576888 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4572370" @default.
• W1913576888 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26201952" @default.
• W1913576888 hasPublicationYear "2015" @default.
• W1913576888 type Work @default.
• W1913576888 sameAs 1913576888 @default.
• W1913576888 citedByCount "11" @default.
• W1913576888 countsByYear W19135768882016 @default.
• W1913576888 countsByYear W19135768882017 @default.
• W1913576888 countsByYear W19135768882018 @default.
• W1913576888 countsByYear W19135768882019 @default.
• W1913576888 countsByYear W19135768882020 @default.
• W1913576888 countsByYear W19135768882022 @default.
• W1913576888 countsByYear W19135768882023 @default.
• W1913576888 crossrefType "journal-article" @default.
• W1913576888 hasAuthorship W1913576888A5032098701 @default.
• W1913576888 hasAuthorship W1913576888A5048563132 @default.
• W1913576888 hasAuthorship W1913576888A5051653179 @default.
• W1913576888 hasAuthorship W1913576888A5062264366 @default.
• W1913576888 hasAuthorship W1913576888A5064973035 @default.
• W1913576888 hasAuthorship W1913576888A5076118562 @default.
• W1913576888 hasAuthorship W1913576888A5077833017 @default.
• W1913576888 hasAuthorship W1913576888A5084394132 @default.
• W1913576888 hasBestOaLocation W19135768881 @default.
• W1913576888 hasConcept C111368507 @default.
• W1913576888 hasConcept C12554922 @default.
• W1913576888 hasConcept C127313418 @default.
• W1913576888 hasConcept C181199279 @default.
• W1913576888 hasConcept C185592680 @default.
• W1913576888 hasConcept C190062978 @default.
• W1913576888 hasConcept C202751555 @default.
• W1913576888 hasConcept C203635412 @default.
• W1913576888 hasConcept C2776879804 @default.
• W1913576888 hasConcept C2776958096 @default.
• W1913576888 hasConcept C2779276759 @default.

• next