FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1914174905> ?p ?o ?g. }

• W1914174905 endingPage "5996" @default.
• W1914174905 startingPage "5989" @default.
• W1914174905 abstract "ABSTRACT Succinate-ubiquinone oxidoreductase (SQR) from Escherichia coli is expressed maximally during aerobic growth, when it catalyzes the oxidation of succinate to fumarate in the tricarboxylic acid cycle and reduces ubiquinone in the membrane. The enzyme is similar in structure and function to fumarate reductase (menaquinol-fumarate oxidoreductase [QFR]), which participates in anaerobic respiration by E. coli . Fumarate reductase, which is proficient in succinate oxidation, is able to functionally replace SQR in aerobic respiration when conditions are used to allow the expression of the frdABCD operon aerobically. SQR has not previously been shown to be capable of supporting anaerobic growth of E. coli because expression of the enzyme complex is largely repressed by anaerobic conditions. In order to obtain expression of SQR anaerobically, plasmids which utilize the P FRD promoter of the frdABCD operon fused to the sdhCDAB genes to drive expression were constructed. It was found that, under anaerobic growth conditions where fumarate is utilized as the terminal electron acceptor, SQR would function to support anaerobic growth of E. coli . The levels of amplification of SQR and QFR were similar under anaerobic growth conditions. The catalytic properties of SQR isolated from anaerobically grown cells were measured and found to be identical to those of enzyme produced aerobically. The anaerobic expression of SQR gave a greater yield of enzyme complex than was found in the membrane from aerobically grown cells under the conditions tested. In addition, it was found that anaerobic expression of SQR could saturate the capacity of the membrane for incorporation of enzyme complex. As has been seen with the amplified QFR complex, E. coli accommodates the excess SQR produced by increasing the amount of membrane. The excess membrane was found in tubular structures that could be seen in thin-section electron micrographs." @default.
• W1914174905 created "2016-06-24" @default.
• W1914174905 creator A5013786719 @default.
• W1914174905 creator A5055800242 @default.
• W1914174905 creator A5060957747 @default.
• W1914174905 date "1998-11-15" @default.
• W1914174905 modified "2023-10-18" @default.
• W1914174905 title "Anaerobic Expression of <i>Escherichia coli</i> Succinate Dehydrogenase: Functional Replacement of Fumarate Reductase in the Respiratory Chain during Anaerobic Growth" @default.
• W1914174905 cites W1152842012 @default.
• W1914174905 cites W1487220623 @default.
• W1914174905 cites W1516181260 @default.
• W1914174905 cites W1541677767 @default.
• W1914174905 cites W1556171318 @default.
• W1914174905 cites W1557366744 @default.
• W1914174905 cites W1563371560 @default.
• W1914174905 cites W1579942088 @default.
• W1914174905 cites W1597763685 @default.
• W1914174905 cites W1599914638 @default.
• W1914174905 cites W1795675295 @default.
• W1914174905 cites W1876175381 @default.
• W1914174905 cites W1969206674 @default.
• W1914174905 cites W1993986279 @default.
• W1914174905 cites W2002807618 @default.
• W1914174905 cites W2012344563 @default.
• W1914174905 cites W2017037513 @default.
• W1914174905 cites W2032703060 @default.
• W1914174905 cites W2035034685 @default.
• W1914174905 cites W2040712576 @default.
• W1914174905 cites W2048114944 @default.
• W1914174905 cites W2067952279 @default.
• W1914174905 cites W2078346397 @default.
• W1914174905 cites W2078397384 @default.
• W1914174905 cites W2085176594 @default.
• W1914174905 cites W2092067755 @default.
• W1914174905 cites W2102860224 @default.
• W1914174905 cites W2104957635 @default.
• W1914174905 cites W2111211778 @default.
• W1914174905 cites W2111627234 @default.
• W1914174905 cites W2406740475 @default.
• W1914174905 cites W7827006 @default.
• W1914174905 cites W88823854 @default.
• W1914174905 doi "https://doi.org/10.1128/jb.180.22.5989-5996.1998" @default.
• W1914174905 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/107675" @default.
• W1914174905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9811659" @default.
• W1914174905 hasPublicationYear "1998" @default.
• W1914174905 type Work @default.
• W1914174905 sameAs 1914174905 @default.
• W1914174905 citedByCount "123" @default.
• W1914174905 countsByYear W19141749052012 @default.
• W1914174905 countsByYear W19141749052013 @default.
• W1914174905 countsByYear W19141749052014 @default.
• W1914174905 countsByYear W19141749052015 @default.
• W1914174905 countsByYear W19141749052016 @default.
• W1914174905 countsByYear W19141749052017 @default.
• W1914174905 countsByYear W19141749052018 @default.
• W1914174905 countsByYear W19141749052019 @default.
• W1914174905 countsByYear W19141749052020 @default.
• W1914174905 countsByYear W19141749052021 @default.
• W1914174905 countsByYear W19141749052022 @default.
• W1914174905 countsByYear W19141749052023 @default.
• W1914174905 crossrefType "journal-article" @default.
• W1914174905 hasAuthorship W1914174905A5013786719 @default.
• W1914174905 hasAuthorship W1914174905A5055800242 @default.
• W1914174905 hasAuthorship W1914174905A5060957747 @default.
• W1914174905 hasBestOaLocation W19141749051 @default.
• W1914174905 hasConcept C104317684 @default.
• W1914174905 hasConcept C121612415 @default.
• W1914174905 hasConcept C134651460 @default.
• W1914174905 hasConcept C165135838 @default.
• W1914174905 hasConcept C181199279 @default.
• W1914174905 hasConcept C203075996 @default.
• W1914174905 hasConcept C24840226 @default.
• W1914174905 hasConcept C2779268744 @default.
• W1914174905 hasConcept C2781330172 @default.
• W1914174905 hasConcept C28859421 @default.
• W1914174905 hasConcept C2900893 @default.
• W1914174905 hasConcept C3020377403 @default.
• W1914174905 hasConcept C42407357 @default.
• W1914174905 hasConcept C48843007 @default.
• W1914174905 hasConcept C5140985 @default.
• W1914174905 hasConcept C547475151 @default.
• W1914174905 hasConcept C55493867 @default.
• W1914174905 hasConcept C66517615 @default.
• W1914174905 hasConcept C86803240 @default.
• W1914174905 hasConceptScore W1914174905C104317684 @default.
• W1914174905 hasConceptScore W1914174905C121612415 @default.
• W1914174905 hasConceptScore W1914174905C134651460 @default.
• W1914174905 hasConceptScore W1914174905C165135838 @default.
• W1914174905 hasConceptScore W1914174905C181199279 @default.
• W1914174905 hasConceptScore W1914174905C203075996 @default.
• W1914174905 hasConceptScore W1914174905C24840226 @default.
• W1914174905 hasConceptScore W1914174905C2779268744 @default.
• W1914174905 hasConceptScore W1914174905C2781330172 @default.
• W1914174905 hasConceptScore W1914174905C28859421 @default.
• W1914174905 hasConceptScore W1914174905C2900893 @default.
• W1914174905 hasConceptScore W1914174905C3020377403 @default.
• W1914174905 hasConceptScore W1914174905C42407357 @default.
• W1914174905 hasConceptScore W1914174905C48843007 @default.

• next