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- W1915744516 abstract "Abstract In this automated system for assaying guanidino compounds, the compounds are resolved on an ion-exchange (Dowex 50) column, then reacted with phenanthrenequinone in alkaline solution. This mixture is then acidified and the resulting fluorescence is measured (lambda ex, 305 nm; lambda em, 395 nm). I applied the system to the analysis of urine from control rats and rats given L-canavanine, L-canavaninosuccinic acid, L-guanidinosuccinic acid, L-arginine, or urea, intraperitoneally. The recorder tracings are compared with those obtained for urine from healthy and uremic humans. After urea administration some guanidinosuccinate is excreted, along with substantial quantities of another substance that elutes just before guanidinosuccinate and so may be mistaken for it. Urine from uremic humans also shows this unidentified peak, but not urine from untreated rats or healthy humans. L-Canavanine gives rise, mainly, to guanidine and homoserine, apparently by reduction. Similarly, canavaninosuccinate is reduced to homoserine and guanidinosuccinate. Arginine gives rise to small quantities of guanidinosuccinate. Guanidinosuccinate is excreted mainly unchanged. When the guanidinosuccinate concentration is increased, excretion of guanidinoacetic acid is suppressed." @default.
- W1915744516 created "2016-06-24" @default.
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- W1915744516 date "1984-02-01" @default.
- W1915744516 modified "2023-10-13" @default.
- W1915744516 title "Metabolic relationship between urea and guanidino compounds as studied by automated fluorimetry of guanidino compounds in urine." @default.
- W1915744516 doi "https://doi.org/10.1093/clinchem/30.2.252" @default.
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