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- W1916028547 abstract "Studies on the development of tissue culture techniques and regeneration of species belonging to the family Cucurbitaceae have been reported (Jelaska, 1986; Chee, 1990). However, reports on regeneration of transformed cucurbits have not been well documented. Trulson et al. (1989) reported the transformation and regeneration of transformed cucumber plants which express the NPT gene. This transformation was achieved using Agrobacterium rhizogenes to transfer the T-DNA region of the binary plasmid pARC8, which contains a Nos-NPT II gene, by infecting cucumber leaf pieces. The resulting transformed roots were regenerated into whole plants (Trulson et al., 1986). The main limitation of this approach is its low frequency of plant regeneration from root tissues. Hence, only a few transformed plants were obtained. The present report describes the use of avirulent strains of Agrobacterium tumefaciens to mediate T-DNA transfers into cucumber cotyledon tissues and the use of somatic embryogenesis route to obtain a high number of morphologically normal transformed plants." @default.
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- W1916028547 date "1990-01-01" @default.
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- W1916028547 title "Transformation of Cucumber Via Agrobacterium Tumefaciens" @default.
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- W1916028547 doi "https://doi.org/10.1007/978-94-009-2103-0_12" @default.
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