Matches in SemOpenAlex for { <https://semopenalex.org/work/W1916089144> ?p ?o ?g. }
- W1916089144 endingPage "650" @default.
- W1916089144 startingPage "636" @default.
- W1916089144 abstract "Although chloroplast protein stability has long been recognised as a major level of post-translational regulation in photosynthesis and gene expression, the factors determining protein stability in plastids are largely unknown. Here, we have identified stability determinants in vivo by producing plants with transgenic chloroplasts that express a reporter protein whose N- and C-termini were systematically modified. We found that major stability determinants are located in the N-terminus. Moreover, testing of all 20 amino acids in the position after the initiator methionine revealed strong differences in protein stability and indicated an important role of the penultimate N-terminal amino acid residue in determining the protein half life. We propose that the stability of plastid proteins is largely determined by three factors: (i) the action of methionine aminopeptidase (the enzyme that removes the initiator methionine and exposes the penultimate N-terminal amino acid residue), (ii) an N-end rule-like protein degradation pathway, and (iii) additional sequence determinants in the N-terminal region." @default.
- W1916089144 created "2016-06-24" @default.
- W1916089144 creator A5048476137 @default.
- W1916089144 creator A5075199511 @default.
- W1916089144 creator A5090816444 @default.
- W1916089144 date "2010-08-01" @default.
- W1916089144 modified "2023-09-26" @default.
- W1916089144 title "Identification of protein stability determinants in chloroplasts" @default.
- W1916089144 cites W11154726 @default.
- W1916089144 cites W1556152083 @default.
- W1916089144 cites W1568454117 @default.
- W1916089144 cites W1703234027 @default.
- W1916089144 cites W1746958425 @default.
- W1916089144 cites W1864022620 @default.
- W1916089144 cites W1897988095 @default.
- W1916089144 cites W1967635084 @default.
- W1916089144 cites W1973987753 @default.
- W1916089144 cites W1976431857 @default.
- W1916089144 cites W1977294998 @default.
- W1916089144 cites W1983345237 @default.
- W1916089144 cites W1985214621 @default.
- W1916089144 cites W1989033034 @default.
- W1916089144 cites W1990148399 @default.
- W1916089144 cites W1991386337 @default.
- W1916089144 cites W1995673848 @default.
- W1916089144 cites W2001096162 @default.
- W1916089144 cites W2004362709 @default.
- W1916089144 cites W2004747515 @default.
- W1916089144 cites W2007866476 @default.
- W1916089144 cites W2009193413 @default.
- W1916089144 cites W2009817687 @default.
- W1916089144 cites W2012265276 @default.
- W1916089144 cites W2013801671 @default.
- W1916089144 cites W2017188875 @default.
- W1916089144 cites W2024556021 @default.
- W1916089144 cites W2028819956 @default.
- W1916089144 cites W2034470900 @default.
- W1916089144 cites W2035569764 @default.
- W1916089144 cites W2038285259 @default.
- W1916089144 cites W2039305280 @default.
- W1916089144 cites W2042397530 @default.
- W1916089144 cites W2043712358 @default.
- W1916089144 cites W2045375219 @default.
- W1916089144 cites W2046347466 @default.
- W1916089144 cites W2049535536 @default.
- W1916089144 cites W2053167380 @default.
- W1916089144 cites W2057842451 @default.
- W1916089144 cites W2067359407 @default.
- W1916089144 cites W2070420713 @default.
- W1916089144 cites W2071958540 @default.
- W1916089144 cites W2075618717 @default.
- W1916089144 cites W2076255097 @default.
- W1916089144 cites W2077817524 @default.
- W1916089144 cites W2082873735 @default.
- W1916089144 cites W2085952533 @default.
- W1916089144 cites W2091248280 @default.
- W1916089144 cites W2093286216 @default.
- W1916089144 cites W2094093683 @default.
- W1916089144 cites W2106765984 @default.
- W1916089144 cites W2107430109 @default.
- W1916089144 cites W2108606795 @default.
- W1916089144 cites W2109777523 @default.
- W1916089144 cites W2110862837 @default.
- W1916089144 cites W2113159943 @default.
- W1916089144 cites W2113180960 @default.
- W1916089144 cites W2114206214 @default.
- W1916089144 cites W2114796662 @default.
- W1916089144 cites W2118705668 @default.
- W1916089144 cites W2119414645 @default.
- W1916089144 cites W2120689646 @default.
- W1916089144 cites W2122366310 @default.
- W1916089144 cites W2122874479 @default.
- W1916089144 cites W2133225212 @default.
- W1916089144 cites W2136144179 @default.
- W1916089144 cites W2136781454 @default.
- W1916089144 cites W2140873409 @default.
- W1916089144 cites W2142260297 @default.
- W1916089144 cites W2144920623 @default.
- W1916089144 cites W2152386425 @default.
- W1916089144 cites W2152490734 @default.
- W1916089144 cites W2162975440 @default.
- W1916089144 cites W2172153643 @default.
- W1916089144 cites W2228914290 @default.
- W1916089144 cites W2576734941 @default.
- W1916089144 cites W29279434 @default.
- W1916089144 doi "https://doi.org/10.1111/j.1365-313x.2010.04268.x" @default.
- W1916089144 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2988409" @default.
- W1916089144 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20545891" @default.
- W1916089144 hasPublicationYear "2010" @default.
- W1916089144 type Work @default.
- W1916089144 sameAs 1916089144 @default.
- W1916089144 citedByCount "104" @default.
- W1916089144 countsByYear W19160891442012 @default.
- W1916089144 countsByYear W19160891442013 @default.
- W1916089144 countsByYear W19160891442014 @default.
- W1916089144 countsByYear W19160891442015 @default.
- W1916089144 countsByYear W19160891442016 @default.
- W1916089144 countsByYear W19160891442017 @default.