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• W1917376029 abstract "An oligosaccharide-lipid containing N -acetyl d -glucosamine (GlcNAc), l -rhamnose, and d -glucose was synthesized when the particulate enzyme from Streptococcus sanguis was incubated with UDP-GlcNAc, TDP-rhamnose, and UDP-glucose. The incorporation of d -glucose into the lipid was dependent on the preincorporation of l -rhamnose, which in turn was dependent on that of GlcNAc. This indicates that the order of sugar incorporation is GlcNAc, l -rhamnose, and d -glucose. The synthesis of GlcNAc-lipid was stimulated twofold by ATP and was inhibited strongly by UDP and slightly by UMP, CDP, and TDP, but not by all other nucleoside diphosphates and nucleoside monophosphates tested. A [γ- 32 P]ATP labeling experiment indicated that some acceptor lipid was present in nonphosphorylated form. The acid and alkaline stabilities of the GlcNAc-lipid were similar to those of glycosyl undecaprenylphosphate, and the thin-layer chromatographic mobility of the lipid was slightly faster than that of the mannosylphosphorylundecaprenol. The molar ratio of phosphate to GlcNAc in purified GlcNAc-lipid was found to be 0.96:1. These results suggested that the GlcNAc was attached to the lipid moiety, presumably undecaprenol, by phosphodiester bonds. The incorporation of l -rhamnose into the lipid was inhibited by UDP and UMP, respectively, in a manner similar to the incorporation of GlcNAc. This suggested that the oligosaccharide was also linked to the lipid moiety by phosphodiester bonds." @default.
• W1917376029 created "2016-06-24" @default.
• W1917376029 creator A5084979328 @default.
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• W1917376029 date "1978-01-01" @default.
• W1917376029 modified "2023-09-26" @default.
• W1917376029 title "Biosynthesis of Oligosaccharide-Lipid in Streptococcus sanguis" @default.
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• W1917376029 doi "https://doi.org/10.1128/jb.133.1.185-195.1978" @default.
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