FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1917777612> ?p ?o ?g. }

• W1917777612 endingPage "2863" @default.
• W1917777612 startingPage "2859" @default.
• W1917777612 abstract "Exopolysaccharides interfere with the isolation and characterization of plasmid DNA from gram-negative bacteria. To repress capsular polysaccharide production, bacteria were cultured in medium containing bismuth nitrate and sodium salicylate. Rapid removal of other contaminating bacterial surface components was achieved by mild acidic zwitterionic detergent extraction. After treatment, bacterial cells were more readily lysed in alkaline detergents. The resulting plasmid preparations contained virtually no capsular polysaccharide and relatively small quantities of lipopolysaccharide and protein, yet they produced yields of nucleic acids similar to those of conventional plasmid preparations. Conventional preparations from encapsulated organisms were largely insoluble and appeared as smears following agarose gel electrophoresis, with indefinite plasmid banding. Plasmids prepared by the new method were highly soluble in conventional buffers and exhibited high-resolution plasmid banding patterns in agarose gels. Plasmids as large as 180 kbp could be isolated and visualized, without apparent nicking, and were readily digested by restriction endonuclease enzymes. The method proved effective with encapsulated or mucoid strains of Klebsiella pneumoniae, Escherichia coli, Acinetobacter anitratus, Salmonella typhimurium, and Enterobacter species. The complete method for plasmid isolation was not suitable for Pseudomonas aeruginosa because of the inhibitory effects of bismuth. Thus, removal of contaminating bacterial surface structures enabled the rapid isolation and characterization of plasmids from mucoid clinical isolates, without the use of organic solvents, CsCl gradients, or expensive, disposable columns." @default.
• W1917777612 created "2016-06-24" @default.
• W1917777612 creator A5001461585 @default.
• W1917777612 creator A5005228659 @default.
• W1917777612 creator A5073305158 @default.
• W1917777612 creator A5074683000 @default.
• W1917777612 date "1992-11-01" @default.
• W1917777612 modified "2023-09-24" @default.
• W1917777612 title "Rapid plasmid DNA isolation from mucoid gram-negative bacteria" @default.
• W1917777612 cites W1548938191 @default.
• W1917777612 cites W1565492579 @default.
• W1917777612 cites W1690413094 @default.
• W1917777612 cites W1775749144 @default.
• W1917777612 cites W1938013375 @default.
• W1917777612 cites W1965028224 @default.
• W1917777612 cites W1976882955 @default.
• W1917777612 cites W1979405597 @default.
• W1917777612 cites W2019410656 @default.
• W1917777612 cites W2032776824 @default.
• W1917777612 cites W2047696108 @default.
• W1917777612 cites W2052788141 @default.
• W1917777612 cites W2068669245 @default.
• W1917777612 cites W2072029917 @default.
• W1917777612 cites W2097912340 @default.
• W1917777612 cites W2130931852 @default.
• W1917777612 cites W2141305167 @default.
• W1917777612 cites W2153179992 @default.
• W1917777612 cites W2166097152 @default.
• W1917777612 cites W2403265727 @default.
• W1917777612 doi "https://doi.org/10.1128/jcm.30.11.2859-2863.1992" @default.
• W1917777612 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/270542" @default.
• W1917777612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1333482" @default.
• W1917777612 hasPublicationYear "1992" @default.
• W1917777612 type Work @default.
• W1917777612 sameAs 1917777612 @default.
• W1917777612 citedByCount "23" @default.
• W1917777612 countsByYear W19177776122012 @default.
• W1917777612 countsByYear W19177776122017 @default.
• W1917777612 countsByYear W19177776122018 @default.
• W1917777612 countsByYear W19177776122021 @default.
• W1917777612 crossrefType "journal-article" @default.
• W1917777612 hasAuthorship W1917777612A5001461585 @default.
• W1917777612 hasAuthorship W1917777612A5005228659 @default.
• W1917777612 hasAuthorship W1917777612A5073305158 @default.
• W1917777612 hasAuthorship W1917777612A5074683000 @default.
• W1917777612 hasBestOaLocation W19177776121 @default.
• W1917777612 hasConcept C104317684 @default.
• W1917777612 hasConcept C109932267 @default.
• W1917777612 hasConcept C116084860 @default.
• W1917777612 hasConcept C128319531 @default.
• W1917777612 hasConcept C205225487 @default.
• W1917777612 hasConcept C22744801 @default.
• W1917777612 hasConcept C24107716 @default.
• W1917777612 hasConcept C2777058267 @default.
• W1917777612 hasConcept C2778271344 @default.
• W1917777612 hasConcept C2778476535 @default.
• W1917777612 hasConcept C523546767 @default.
• W1917777612 hasConcept C54355233 @default.
• W1917777612 hasConcept C547475151 @default.
• W1917777612 hasConcept C552990157 @default.
• W1917777612 hasConcept C55493867 @default.
• W1917777612 hasConcept C86803240 @default.
• W1917777612 hasConcept C89423630 @default.
• W1917777612 hasConceptScore W1917777612C104317684 @default.
• W1917777612 hasConceptScore W1917777612C109932267 @default.
• W1917777612 hasConceptScore W1917777612C116084860 @default.
• W1917777612 hasConceptScore W1917777612C128319531 @default.
• W1917777612 hasConceptScore W1917777612C205225487 @default.
• W1917777612 hasConceptScore W1917777612C22744801 @default.
• W1917777612 hasConceptScore W1917777612C24107716 @default.
• W1917777612 hasConceptScore W1917777612C2777058267 @default.
• W1917777612 hasConceptScore W1917777612C2778271344 @default.
• W1917777612 hasConceptScore W1917777612C2778476535 @default.
• W1917777612 hasConceptScore W1917777612C523546767 @default.
• W1917777612 hasConceptScore W1917777612C54355233 @default.
• W1917777612 hasConceptScore W1917777612C547475151 @default.
• W1917777612 hasConceptScore W1917777612C552990157 @default.
• W1917777612 hasConceptScore W1917777612C55493867 @default.
• W1917777612 hasConceptScore W1917777612C86803240 @default.
• W1917777612 hasConceptScore W1917777612C89423630 @default.
• W1917777612 hasIssue "11" @default.
• W1917777612 hasLocation W19177776121 @default.
• W1917777612 hasLocation W19177776122 @default.
• W1917777612 hasOpenAccess W1917777612 @default.
• W1917777612 hasPrimaryLocation W19177776121 @default.
• W1917777612 hasRelatedWork W1498218096 @default.
• W1917777612 hasRelatedWork W1510612702 @default.
• W1917777612 hasRelatedWork W152066294 @default.
• W1917777612 hasRelatedWork W1581621822 @default.
• W1917777612 hasRelatedWork W1725170570 @default.
• W1917777612 hasRelatedWork W178028534 @default.
• W1917777612 hasRelatedWork W1968694237 @default.
• W1917777612 hasRelatedWork W1977053519 @default.
• W1917777612 hasRelatedWork W2007583941 @default.
• W1917777612 hasRelatedWork W2018679173 @default.
• W1917777612 hasRelatedWork W2019410656 @default.
• W1917777612 hasRelatedWork W2032978339 @default.
• W1917777612 hasRelatedWork W2044427767 @default.

• next