FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1917967503> ?p ?o ?g. }

• W1917967503 endingPage "1552" @default.
• W1917967503 startingPage "1542" @default.
• W1917967503 abstract "BACKGROUND AND PURPOSE Caffeine is consumed extensively in Europe and North America. As a risk factor for osteoporosis, epidemiological studies have observed that caffeine can decrease bone mineral density, adversely affect calcium absorption and increase the risk of bone fracture. However, the exact mechanisms have not been fully investigated. Here, we examined the effects of caffeine on the viability and osteogenesis of rat bone marrow‐derived mesenchymal stromal cells (rBMSCs). EXPERIMENTAL APPROACH Cell viability, apoptosis and necrosis were quantified using thymidine incorporation and flow cytometry. Sequential gene expressions in osteogenic process were measured by real‐time PCR. cAMP, alkaline phosphatase and osteocalcin were assessed by immunoassay, spectrophotometry and radioimmunoassay, respectively. Mineralization was determined by calcium deposition. KEY RESULTS After treating BMSCs with high caffeine concentrations (0.1–1 mM), their viability decreased in a concentration‐dependent manner. This cell death was primarily due to necrosis and, to a small extent, apoptosis. Genes and protein sequentially expressed in osteogenesis, including Cbfa1/Runx2, collagen I, alkaline phosphatase and its protein, were significantly downregulated except for osteocalcin and its protein. Moreover, caffeine inhibited calcium deposition in a concentration‐ and time‐dependent manner, but increased intracellular cAMP in a concentration‐dependent manner. CONCLUSIONS AND IMPLICATIONS By suppressing the commitment of BMSCs to the osteogenic lineage and selectively inhibiting gene expression, caffeine downregulated some important events in osteogenesis and ultimately affected bone mass." @default.
• W1917967503 created "2016-06-24" @default.
• W1917967503 creator A5011650519 @default.
• W1917967503 creator A5019840630 @default.
• W1917967503 creator A5026656473 @default.
• W1917967503 creator A5057348664 @default.
• W1917967503 creator A5084055594 @default.
• W1917967503 creator A5084733831 @default.
• W1917967503 creator A5088304215 @default.
• W1917967503 date "2010-11-05" @default.
• W1917967503 modified "2023-10-17" @default.
• W1917967503 title "Caffeine inhibits the viability and osteogenic differentiation of rat bone marrow-derived mesenchymal stromal cells" @default.
• W1917967503 cites W1907784997 @default.
• W1917967503 cites W1912594399 @default.
• W1917967503 cites W1972285228 @default.
• W1917967503 cites W1978737037 @default.
• W1917967503 cites W1985148410 @default.
• W1917967503 cites W1991829844 @default.
• W1917967503 cites W1994596418 @default.
• W1917967503 cites W1997812904 @default.
• W1917967503 cites W2002320509 @default.
• W1917967503 cites W2004050413 @default.
• W1917967503 cites W2006950202 @default.
• W1917967503 cites W2014979591 @default.
• W1917967503 cites W2032752377 @default.
• W1917967503 cites W2034315581 @default.
• W1917967503 cites W2040057440 @default.
• W1917967503 cites W2074571303 @default.
• W1917967503 cites W2079404795 @default.
• W1917967503 cites W2081894268 @default.
• W1917967503 cites W2089454086 @default.
• W1917967503 cites W2097067231 @default.
• W1917967503 cites W2106180679 @default.
• W1917967503 cites W2114416716 @default.
• W1917967503 cites W2114528551 @default.
• W1917967503 cites W2119774673 @default.
• W1917967503 cites W2124566101 @default.
• W1917967503 cites W2126482839 @default.
• W1917967503 cites W2128639917 @default.
• W1917967503 cites W2130528241 @default.
• W1917967503 cites W2137298996 @default.
• W1917967503 cites W2139785110 @default.
• W1917967503 cites W2142256883 @default.
• W1917967503 cites W2143334477 @default.
• W1917967503 cites W2144268541 @default.
• W1917967503 cites W2159443200 @default.
• W1917967503 cites W2171160817 @default.
• W1917967503 cites W2323830513 @default.
• W1917967503 cites W4238168677 @default.
• W1917967503 cites W4248939352 @default.
• W1917967503 cites W4251302656 @default.
• W1917967503 doi "https://doi.org/10.1111/j.1476-5381.2010.00998.x" @default.
• W1917967503 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3010566" @default.
• W1917967503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20726981" @default.
• W1917967503 hasPublicationYear "2010" @default.
• W1917967503 type Work @default.
• W1917967503 sameAs 1917967503 @default.
• W1917967503 citedByCount "44" @default.
• W1917967503 countsByYear W19179675032012 @default.
• W1917967503 countsByYear W19179675032013 @default.
• W1917967503 countsByYear W19179675032014 @default.
• W1917967503 countsByYear W19179675032015 @default.
• W1917967503 countsByYear W19179675032016 @default.
• W1917967503 countsByYear W19179675032017 @default.
• W1917967503 countsByYear W19179675032018 @default.
• W1917967503 countsByYear W19179675032019 @default.
• W1917967503 countsByYear W19179675032020 @default.
• W1917967503 countsByYear W19179675032021 @default.
• W1917967503 countsByYear W19179675032022 @default.
• W1917967503 crossrefType "journal-article" @default.
• W1917967503 hasAuthorship W1917967503A5011650519 @default.
• W1917967503 hasAuthorship W1917967503A5019840630 @default.
• W1917967503 hasAuthorship W1917967503A5026656473 @default.
• W1917967503 hasAuthorship W1917967503A5057348664 @default.
• W1917967503 hasAuthorship W1917967503A5084055594 @default.
• W1917967503 hasAuthorship W1917967503A5084733831 @default.
• W1917967503 hasAuthorship W1917967503A5088304215 @default.
• W1917967503 hasBestOaLocation W19179675032 @default.
• W1917967503 hasConcept C126322002 @default.
• W1917967503 hasConcept C131075544 @default.
• W1917967503 hasConcept C134018914 @default.
• W1917967503 hasConcept C160160445 @default.
• W1917967503 hasConcept C16930146 @default.
• W1917967503 hasConcept C181199279 @default.
• W1917967503 hasConcept C185592680 @default.
• W1917967503 hasConcept C190283241 @default.
• W1917967503 hasConcept C198826908 @default.
• W1917967503 hasConcept C202751555 @default.
• W1917967503 hasConcept C2776363554 @default.
• W1917967503 hasConcept C2778260815 @default.
• W1917967503 hasConcept C2778500429 @default.
• W1917967503 hasConcept C2780007613 @default.
• W1917967503 hasConcept C519063684 @default.
• W1917967503 hasConcept C53227056 @default.
• W1917967503 hasConcept C55493867 @default.
• W1917967503 hasConcept C71924100 @default.
• W1917967503 hasConcept C86803240 @default.
• W1917967503 hasConcept C95444343 @default.

• next