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• W1918151242 abstract "Transposable elements (TEs) and RNA interference (RNAi) are excellent genetic tools that could help control the incidence and spread of mosquito-borne diseases such as malaria, dengue, yellow fever, etc. This study aimed to 1) understand the RNAi mechanism in Culex quinquefasciatus, which will be used to study genes involved in pathogen transmission and will help reveal role of RNAi in antiviral immunity in this species; and 2) characterize the DNA sequences that regulate Herves transposase binding, which will help us understand its pre- and post-integration behavior within the host. By using the white eye-pigmentation gene as a marker for RNAi function we demonstrated that introducing dsRNA into embryos of Cx. quinquefasciatus induces a specific functional RNAi response, which silenced the white gene, consequently allowing a white-eye phenotype to be produced in the hatched larvae and adults. Sequence-specific knockdown of key RNAi components was achieved by introducing the homologous dsRNA into embryos of Cx. quinquefasciatus. We found ago2-mediated slicing to be more critical than dcr2-mediated dicing for the functional RNAi response in Cx. quinquefasciatus. Our phylogenetic analysis confirmed the previously reported results that RNAi components for small RNA biogenesis are present in Cx. quinquefasciatus. In addition, we found important differences in the number and the expression of ago genes, the predicted domain architecture of Dcr, and the RNAi response between Cx. quinquefasciatus and the model organism D. melanogaster. Transposition of Class II TEs is regulated by both cis-acting sequences and trans-acting host factors. In this study, we used purified Herves transposase to characterize the specific DNA-binding sites of the Herves transposase. The purified active Herves transposase showed site-specific binding to the subterminal and terminal sequences of the L- and R- ends of the element, respectively. Furthermore, the transposase bound strongly with the R-TIR but failed to bind to the L-TIR. We identified an 8bp sequence repeat as the transposase binding motif that is conserved on both the L- and R-end sequences and is critical and sufficient for Herves transposase binding." @default.
• W1918151242 created "2016-06-24" @default.
• W1918151242 creator A5059139721 @default.
• W1918151242 date "2010-01-01" @default.
• W1918151242 modified "2023-09-27" @default.
• W1918151242 title "In vitro Study of Herves Transposable Element of Anopheles gambiae and Use of RNA Interference (RNAi) in Culex quinquefasciatus" @default.
• W1918151242 cites W1516118238 @default.
• W1918151242 cites W1534179616 @default.
• W1918151242 cites W1611419331 @default.
• W1918151242 cites W1647075334 @default.
• W1918151242 cites W1734591894 @default.
• W1918151242 cites W1860733958 @default.
• W1918151242 cites W1899701789 @default.
• W1918151242 cites W1963708619 @default.
• W1918151242 cites W1965747854 @default.
• W1918151242 cites W1967515061 @default.
• W1918151242 cites W1967633838 @default.
• W1918151242 cites W1969034482 @default.
• W1918151242 cites W1970603979 @default.
• W1918151242 cites W1972649540 @default.
• W1918151242 cites W1972824210 @default.
• W1918151242 cites W1974465530 @default.
• W1918151242 cites W1976342457 @default.
• W1918151242 cites W1976879480 @default.
• W1918151242 cites W1979350018 @default.
• W1918151242 cites W1980269624 @default.
• W1918151242 cites W1982828686 @default.
• W1918151242 cites W1982944995 @default.
• W1918151242 cites W1988103275 @default.
• W1918151242 cites W1988233506 @default.
• W1918151242 cites W1989759946 @default.
• W1918151242 cites W1991007428 @default.
• W1918151242 cites W1991894986 @default.
• W1918151242 cites W1992386924 @default.
• W1918151242 cites W1993881548 @default.
• W1918151242 cites W1993889815 @default.
• W1918151242 cites W1994528729 @default.
• W1918151242 cites W1995004686 @default.
• W1918151242 cites W1995511925 @default.
• W1918151242 cites W1995677771 @default.
• W1918151242 cites W1996065891 @default.
• W1918151242 cites W1997191014 @default.
• W1918151242 cites W1997412021 @default.
• W1918151242 cites W2003330861 @default.
• W1918151242 cites W2004744868 @default.
• W1918151242 cites W2006293480 @default.
• W1918151242 cites W2007793421 @default.
• W1918151242 cites W2008276103 @default.
• W1918151242 cites W2008716207 @default.
• W1918151242 cites W2009207290 @default.
• W1918151242 cites W2010419186 @default.
• W1918151242 cites W2011816659 @default.
• W1918151242 cites W2011981585 @default.
• W1918151242 cites W2020449810 @default.
• W1918151242 cites W2024367691 @default.
• W1918151242 cites W2024829095 @default.
• W1918151242 cites W2026519705 @default.
• W1918151242 cites W2029433700 @default.
• W1918151242 cites W2030313559 @default.
• W1918151242 cites W2030489085 @default.
• W1918151242 cites W2033096908 @default.
• W1918151242 cites W2033518550 @default.
• W1918151242 cites W2034255392 @default.
• W1918151242 cites W2036868291 @default.
• W1918151242 cites W2036868716 @default.
• W1918151242 cites W2036994672 @default.
• W1918151242 cites W2038081057 @default.
• W1918151242 cites W2039718244 @default.
• W1918151242 cites W2042641363 @default.
• W1918151242 cites W2043283675 @default.
• W1918151242 cites W2043698583 @default.
• W1918151242 cites W2043886194 @default.
• W1918151242 cites W2044193703 @default.
• W1918151242 cites W2045739426 @default.
• W1918151242 cites W2045869260 @default.
• W1918151242 cites W2050228332 @default.
• W1918151242 cites W2050557699 @default.
• W1918151242 cites W2051252016 @default.
• W1918151242 cites W2053350421 @default.
• W1918151242 cites W2054156026 @default.
• W1918151242 cites W2057072047 @default.
• W1918151242 cites W2058193823 @default.
• W1918151242 cites W2069799979 @default.
• W1918151242 cites W2071441005 @default.
• W1918151242 cites W2077166550 @default.
• W1918151242 cites W2080252841 @default.
• W1918151242 cites W2080459765 @default.
• W1918151242 cites W2085465281 @default.
• W1918151242 cites W2088262304 @default.
• W1918151242 cites W2089681390 @default.
• W1918151242 cites W2090437002 @default.
• W1918151242 cites W2093008096 @default.
• W1918151242 cites W2100932663 @default.
• W1918151242 cites W2102129274 @default.
• W1918151242 cites W2103557260 @default.
• W1918151242 cites W2108244474 @default.
• W1918151242 cites W2108314707 @default.
• W1918151242 cites W2109942612 @default.
• W1918151242 cites W2110637060 @default.
• W1918151242 cites W2110925400 @default.

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