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• W1918317419 startingPage "418" @default.
• W1918317419 abstract "A simple, rapid mixed agglutination test using sheep erythrocytes (SRBC) sensitized with rabbit hemolysin and intact viable staphylococci is described for the detection of bound staphylococcal protein A. Soluble protein A was heat extracted from 50 clinical isolates as well as the Cowan I and Wood 46 strains of Staphylococcus aureus and titered by a hemagglutination test using sensitized SRBC and dilutions of soluble protein A. Protein A could be detected in all of these supernatants including that of S. aureus Wood 46, a strain generally considered to be protein A negative. These organisms were later retested by the mixed agglutination test and even those staphylococcal isolates expressing very low heat-extractable soluble protein A concentrations (1:2 titers) were positive, confirming the sensitivity of the test. In a screen of clinical isolates, only 4 of 235 (1.8%) coagulase-positive isolates were negative in the mixed agglutination test. Of 25 coagulase-negative isolates, none yielded a positive reaction." @default.
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• W1918317419 date "1976-11-01" @default.
• W1918317419 modified "2023-10-18" @default.
• W1918317419 title "Single-tube mixed agglutination test for the detection of staphylococcal protein A" @default.
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• W1918317419 doi "https://doi.org/10.1128/jcm.4.5.418-422.1976" @default.
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