FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1918416206> ?p ?o ?g. }

• W1918416206 endingPage "300" @default.
• W1918416206 startingPage "294" @default.
• W1918416206 abstract "Abstract N G , N G ‐dimethyl‐ l ‐arginine (asymmetric dimethylarginine, ADMA), N G ‐monomethyl‐ l ‐arginine ( l ‐NMMA) and N G , N G ’ ‐dimethyl‐ l ‐arginine (symmetric dimethylarginine, SDMA) are released during hydrolysis of proteins containing methylated arginine residues. ADMA and l ‐NMMA inhibit nitric oxide synthase by competing with l ‐arginine substrate. All three methylarginine derivatives also inhibit arginine transport. To enable investigation of methylarginines in diseases involving impaired nitric oxide synthesis, we developed a high‐performance liquid chromatography (HPLC) assay to simultaneously quantify arginine, ADMA, l ‐NMMA and SDMA. Our assay requires 12 μL of plasma and is ideal for applications where sample availability is limited. We extracted arginine and methylarginines with mixed‐mode cation‐exchange columns, using synthetic monoethyl‐ l ‐arginine as an internal standard. Metabolites were derivatized with ortho‐phthaldialdeyhde and 3‐mercaptopropionic acid, separated by reverse‐phase HPLC and quantified with fluorescence detection. Standard curve linearity was ≥0.9995 for all metabolites. Inter‐day coefficient of variation (CV) values were ≤5% for arginine, ADMA and SDMA in human plasma and for arginine and ADMA in mouse plasma. The CV value for l ‐NMMA was higher in human (10.4%) and mouse (15.8%) plasma because concentrations were substantially lower than ADMA and SDMA. This assay provides unique advantages of small sample volume requirements, excellent separation of target metabolites from contaminants and validation for both human and mouse plasma samples. © 2015 The Authors Biomedical Chromatography published by John Wiley & Sons, Ltd." @default.
• W1918416206 created "2016-06-24" @default.
• W1918416206 creator A5019122570 @default.
• W1918416206 creator A5052889798 @default.
• W1918416206 creator A5063241324 @default.
• W1918416206 creator A5081848912 @default.
• W1918416206 date "2015-07-30" @default.
• W1918416206 modified "2023-10-06" @default.
• W1918416206 title "Resolution and quantification of arginine, monomethylarginine, asymmetric dimethylarginine, and symmetric dimethylarginine in plasma using HPLC with internal calibration" @default.
• W1918416206 cites W1899322432 @default.
• W1918416206 cites W1974358274 @default.
• W1918416206 cites W1976085800 @default.
• W1918416206 cites W1977844791 @default.
• W1918416206 cites W1980228925 @default.
• W1918416206 cites W1982589778 @default.
• W1918416206 cites W1990025317 @default.
• W1918416206 cites W2004837312 @default.
• W1918416206 cites W2007778278 @default.
• W1918416206 cites W2008201148 @default.
• W1918416206 cites W2021018646 @default.
• W1918416206 cites W2025293337 @default.
• W1918416206 cites W2031027459 @default.
• W1918416206 cites W2037655806 @default.
• W1918416206 cites W2040643466 @default.
• W1918416206 cites W2052240447 @default.
• W1918416206 cites W2052631825 @default.
• W1918416206 cites W2058385357 @default.
• W1918416206 cites W2061976121 @default.
• W1918416206 cites W2062323653 @default.
• W1918416206 cites W2066583469 @default.
• W1918416206 cites W2073133399 @default.
• W1918416206 cites W2081378069 @default.
• W1918416206 cites W2088632030 @default.
• W1918416206 cites W2106422918 @default.
• W1918416206 cites W2106478344 @default.
• W1918416206 cites W2145802142 @default.
• W1918416206 cites W2148531805 @default.
• W1918416206 cites W2158676184 @default.
• W1918416206 cites W2166937516 @default.
• W1918416206 cites W2169513794 @default.
• W1918416206 cites W2062801753 @default.
• W1918416206 doi "https://doi.org/10.1002/bmc.3548" @default.
• W1918416206 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4755038" @default.
• W1918416206 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26130049" @default.
• W1918416206 hasPublicationYear "2015" @default.
• W1918416206 type Work @default.
• W1918416206 sameAs 1918416206 @default.
• W1918416206 citedByCount "8" @default.
• W1918416206 countsByYear W19184162062017 @default.
• W1918416206 countsByYear W19184162062019 @default.
• W1918416206 countsByYear W19184162062020 @default.
• W1918416206 countsByYear W19184162062021 @default.
• W1918416206 countsByYear W19184162062023 @default.
• W1918416206 crossrefType "journal-article" @default.
• W1918416206 hasAuthorship W1918416206A5019122570 @default.
• W1918416206 hasAuthorship W1918416206A5052889798 @default.
• W1918416206 hasAuthorship W1918416206A5063241324 @default.
• W1918416206 hasAuthorship W1918416206A5081848912 @default.
• W1918416206 hasBestOaLocation W19184162061 @default.
• W1918416206 hasConcept C119128265 @default.
• W1918416206 hasConcept C178790620 @default.
• W1918416206 hasConcept C179998833 @default.
• W1918416206 hasConcept C185592680 @default.
• W1918416206 hasConcept C23531484 @default.
• W1918416206 hasConcept C2777468819 @default.
• W1918416206 hasConcept C2779877776 @default.
• W1918416206 hasConcept C43617362 @default.
• W1918416206 hasConcept C515207424 @default.
• W1918416206 hasConcept C519581460 @default.
• W1918416206 hasConcept C55493867 @default.
• W1918416206 hasConceptScore W1918416206C119128265 @default.
• W1918416206 hasConceptScore W1918416206C178790620 @default.
• W1918416206 hasConceptScore W1918416206C179998833 @default.
• W1918416206 hasConceptScore W1918416206C185592680 @default.
• W1918416206 hasConceptScore W1918416206C23531484 @default.
• W1918416206 hasConceptScore W1918416206C2777468819 @default.
• W1918416206 hasConceptScore W1918416206C2779877776 @default.
• W1918416206 hasConceptScore W1918416206C43617362 @default.
• W1918416206 hasConceptScore W1918416206C515207424 @default.
• W1918416206 hasConceptScore W1918416206C519581460 @default.
• W1918416206 hasConceptScore W1918416206C55493867 @default.
• W1918416206 hasIssue "3" @default.
• W1918416206 hasLocation W19184162061 @default.
• W1918416206 hasLocation W19184162062 @default.
• W1918416206 hasLocation W19184162063 @default.
• W1918416206 hasOpenAccess W1918416206 @default.
• W1918416206 hasPrimaryLocation W19184162061 @default.
• W1918416206 hasRelatedWork W1997545423 @default.
• W1918416206 hasRelatedWork W2010799584 @default.
• W1918416206 hasRelatedWork W2103425018 @default.
• W1918416206 hasRelatedWork W2152615286 @default.
• W1918416206 hasRelatedWork W2153125904 @default.
• W1918416206 hasRelatedWork W2328898609 @default.
• W1918416206 hasRelatedWork W2463957749 @default.
• W1918416206 hasRelatedWork W2915113681 @default.
• W1918416206 hasRelatedWork W4293776191 @default.
• W1918416206 hasRelatedWork W72340863 @default.
• W1918416206 hasVolume "30" @default.

• next