FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1919680839> ?p ?o ?g. }

• W1919680839 abstract "Legionella pneumophila has high iron requirements, and its intracellular growth in human monocytes is dependent on the availability of intracellular iron. To learn more about iron metabolism in L. pneumophila, we have undertaken an analysis of the iron proteins of the bacterium. We first developed an assay to identify proteins by 59Fe labelling and nondenaturing polyacrylamide gel electrophoresis. The assay revealed seven iron proteins (IPs) with apparent molecular weights of 500, 450, 250, 210, 150, 130, and 85. IP150 comigrates with superoxide dismutase activity and is probably the Fe-superoxide dismutase of L. pneumophila. IP210 is the major iron-containing protein (MICP). To identify and characterize MICP, we purified the protein and cloned and sequenced its gene. MICP is a monomeric protein containing 891 amino acids, and it has a calculated molecular mass of 98,147 Da. Analysis of the sequence revealed that MICP has two interesting homologies. First, MICP is highly homologous with the human iron-responsive element-binding protein, consistent with the hypothesis that this critical iron-regulatory molecule of humans has a prokaryotic ancestor. Second, MICP is highly homologous with the Escherichia coli aconitase and to a lesser extent with porcine heart mitochondrial aconitase. Consistent with this, we found that MICP exhibits aconitase activity. In contrast to other aconitases, MICP has a single amino acid change of a potentially deleterious type at a site thought to be critical for substrate binding and enzymatic activity. However, the specific activity of MICP is roughly comparable to that of other aconitases, suggesting that the mutation has at most a mild effect on the aconitase activity of MICP. The abundance of MICP in L. pneumophila suggests either that L. pneumophila requires high aconitase and perhaps tricarboxylic acid cycle activity or that the bacterium requires large amounts of this protein to serve an additional role in bacterial physiology. A need for large amounts of MICP, which contains four Fe atoms per molecule when fully loaded, could at least partly explain L. pneumophila's high metabolic requirement for iron." @default.
• W1919680839 created "2016-06-24" @default.
• W1919680839 creator A5044071246 @default.
• W1919680839 creator A5062026780 @default.
• W1919680839 date "1993-09-01" @default.
• W1919680839 modified "2023-10-17" @default.
• W1919680839 title "The major iron-containing protein of Legionella pneumophila is an aconitase homologous with the human iron-responsive element-binding protein" @default.
• W1919680839 cites W149801576 @default.
• W1919680839 cites W1501405625 @default.
• W1919680839 cites W1516318762 @default.
• W1919680839 cites W1525935121 @default.
• W1919680839 cites W1555038407 @default.
• W1919680839 cites W1561975639 @default.
• W1919680839 cites W1565459224 @default.
• W1919680839 cites W1592463355 @default.
• W1919680839 cites W1596833660 @default.
• W1919680839 cites W1794036758 @default.
• W1919680839 cites W1877825715 @default.
• W1919680839 cites W1922804342 @default.
• W1919680839 cites W1932655858 @default.
• W1919680839 cites W1947746754 @default.
• W1919680839 cites W1952522348 @default.
• W1919680839 cites W1969206674 @default.
• W1919680839 cites W1969289450 @default.
• W1919680839 cites W1969383250 @default.
• W1919680839 cites W1971099877 @default.
• W1919680839 cites W1973968247 @default.
• W1919680839 cites W1976085354 @default.
• W1919680839 cites W1980890795 @default.
• W1919680839 cites W1999347013 @default.
• W1919680839 cites W1999691755 @default.
• W1919680839 cites W2000559951 @default.
• W1919680839 cites W2009310436 @default.
• W1919680839 cites W2010883644 @default.
• W1919680839 cites W2013297780 @default.
• W1919680839 cites W2014039656 @default.
• W1919680839 cites W2017302711 @default.
• W1919680839 cites W2021231178 @default.
• W1919680839 cites W2030447631 @default.
• W1919680839 cites W2031121939 @default.
• W1919680839 cites W2054543860 @default.
• W1919680839 cites W2060703599 @default.
• W1919680839 cites W2067527827 @default.
• W1919680839 cites W2068022490 @default.
• W1919680839 cites W2070009162 @default.
• W1919680839 cites W2071769587 @default.
• W1919680839 cites W2078902479 @default.
• W1919680839 cites W2082017984 @default.
• W1919680839 cites W2090979926 @default.
• W1919680839 cites W2094794452 @default.
• W1919680839 cites W2101122522 @default.
• W1919680839 cites W2101422765 @default.
• W1919680839 cites W2102689412 @default.
• W1919680839 cites W2116029568 @default.
• W1919680839 cites W2119711133 @default.
• W1919680839 cites W2120502960 @default.
• W1919680839 cites W2130904512 @default.
• W1919680839 cites W2147171214 @default.
• W1919680839 cites W2151805768 @default.
• W1919680839 cites W2154535574 @default.
• W1919680839 cites W2162573539 @default.
• W1919680839 cites W2164683251 @default.
• W1919680839 cites W2253080030 @default.
• W1919680839 cites W2260710019 @default.
• W1919680839 cites W241460695 @default.
• W1919680839 cites W299581075 @default.
• W1919680839 doi "https://doi.org/10.1128/jb.175.17.5666-5676.1993" @default.
• W1919680839 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/206625" @default.
• W1919680839 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8366052" @default.
• W1919680839 hasPublicationYear "1993" @default.
• W1919680839 type Work @default.
• W1919680839 sameAs 1919680839 @default.
• W1919680839 citedByCount "55" @default.
• W1919680839 countsByYear W19196808392014 @default.
• W1919680839 countsByYear W19196808392015 @default.
• W1919680839 crossrefType "journal-article" @default.
• W1919680839 hasAuthorship W1919680839A5044071246 @default.
• W1919680839 hasAuthorship W1919680839A5062026780 @default.
• W1919680839 hasBestOaLocation W19196808391 @default.
• W1919680839 hasConcept C101660505 @default.
• W1919680839 hasConcept C104317684 @default.
• W1919680839 hasConcept C116084860 @default.
• W1919680839 hasConcept C153911025 @default.
• W1919680839 hasConcept C167625842 @default.
• W1919680839 hasConcept C181199279 @default.
• W1919680839 hasConcept C200886947 @default.
• W1919680839 hasConcept C2776118409 @default.
• W1919680839 hasConcept C2779369046 @default.
• W1919680839 hasConcept C515207424 @default.
• W1919680839 hasConcept C523546767 @default.
• W1919680839 hasConcept C54355233 @default.
• W1919680839 hasConcept C547475151 @default.
• W1919680839 hasConcept C55493867 @default.
• W1919680839 hasConcept C58121356 @default.
• W1919680839 hasConcept C86803240 @default.
• W1919680839 hasConceptScore W1919680839C101660505 @default.
• W1919680839 hasConceptScore W1919680839C104317684 @default.
• W1919680839 hasConceptScore W1919680839C116084860 @default.
• W1919680839 hasConceptScore W1919680839C153911025 @default.
• W1919680839 hasConceptScore W1919680839C167625842 @default.

• next