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- W1919850818 abstract "Abstract This unit presents two basic protocols to determine adenylate cyclase and guanylate cyclase activity in tissue and cell homogenates, permeabilized cells, or subcellular fractions. Two parts are presented for each method. First, the enzyme reaction that causes the formation of the labeled cyclic nucleotide is performed. Second, the separation of cyclic nucleotide products from unreacted nucleotide triphosphates and metabolites is performed using Dowex 50 resin and aluminum oxide chromatographies. In the case of guanylate cyclase, alternative separation protocols are also provided. Additionally, support protocols are provided that describe both the preparation of columns used in the assays and the preparation of tissue or cells to be assayed." @default.
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- W1919850818 date "1998-03-01" @default.
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- W1919850818 title "Adenylate and Guanylate Cyclase Assays" @default.
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- W1919850818 doi "https://doi.org/10.1002/0471141755.ph0202s00" @default.
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