Matches in SemOpenAlex for { <https://semopenalex.org/work/W1920044544> ?p ?o ?g. }
- W1920044544 abstract "PCR primers are available for virtually every region of the plastid genome. Selection of which primer pairs to use is second only to selection of the genic region. This is particularly true for research at the species/population interface.Primer pairs for 130 regions of the chloroplast genome were evaluated in 12 species distributed across the angiosperms. Likelihood of amplification success was inferred based upon number and location of mismatches to target sequence. Intraspecific sequence variability was evaluated under three different criteria in four species.Many published primer pairs should work across all taxa sampled, with the exception of failure due to genomic reorganization events. Universal barcoding primers were the least likely to work (65% success). The list of most variable regions for use within species has little in common with the lists identified in prior studies.Published primer sequences should amplify a diversity of flowering plant DNAs, even those designed for specific taxonomic groups. Universal primers may have extremely limited utility. There was little consistency in likelihood of amplification success for any given publication across lineages or within lineage across publications." @default.
- W1920044544 created "2016-06-24" @default.
- W1920044544 creator A5052578797 @default.
- W1920044544 date "2015-06-01" @default.
- W1920044544 modified "2023-10-01" @default.
- W1920044544 title "Plastid Primers for Angiosperm Phylogenetics and Phylogeography" @default.
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- W1920044544 doi "https://doi.org/10.3732/apps.1400085" @default.
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