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• W1920473032 abstract "The alveolar epithelium maintains lung fluid homeostasis by active ion transport. The molecularmachinery required to create an osmotic gradient across the alveolar epithelium, which in turnmoves water out of the airspaces, has been identified by number of experimental techniques.The alveolar epithelium, which covers 95% of the surface area of the lung, is composed of twomorphologically distinct epithelial cell types: alveolar epithelial type one and alveolar epithelialtype two cells. The much studied alveolar epithelial type two cell, which cover <5% of thesurface, contain the myriad of ion channel and transports required to undertake fluid transport.The alveolar epithelial type one cell, which covers >95% of the surface area, is less wellcharacterised. Direct functional electrophysiological measurements from the alveolar epithelialtype one cell has proved difficult to obtain.In order to obtain novel recordings the unfilled lung slice experimental model was developed.This experimental model demonstrated excellent cellular viability. VIIIB2 mouse monoclonalantibody, a specific cell marker for alveolar epithelial type one cells, was used to obtain live cellimmunohistochemistry, the first such reported in peripheral tissue. The results presented in thisthesis represent the first published ion channel recordings from identified alveolar epithelial typeone cells within a rat lung slice (Bourke, S. et al 2005). Cell-attached, patch-clamp recordingsdemonstrated the presence of a voltage-dependant, K+ selective ion channel with a measuredunitary conductance of 21 pS. The findings contained within this thesis present a novelexperimental technique to investigate the functional physiological role of the alveolar epithelialcells.The alveolar epithelium is reliant upon Ca2+ signalling mechanisms to undertake a number ofnormal physiological activities. The release of surfactant from the alveolar epithelial type two celllamellar bodies relies on a Ca2+ dependant exocytosis step. To investigate further the function ofCa2+ in the alveolar cells, experiments were undertaken using Ca2+ fluorescent indicator dyeFluo-3. Slices demonstrated spontaneous Ca2+ oscillations. Application of a number ofphysiological and pharmacological substances failed to modulate the observed activity." @default.
• W1920473032 created "2016-06-24" @default.
• W1920473032 creator A5086152654 @default.
• W1920473032 date "2010-01-01" @default.
• W1920473032 modified "2023-09-27" @default.
• W1920473032 title "Investigation of ion channel activity in alveolarepithelial cells.The development of lung slice in-vitro model." @default.
• W1920473032 cites W1502147118 @default.
• W1920473032 cites W1506387083 @default.
• W1920473032 cites W1517576768 @default.
• W1920473032 cites W1529590189 @default.
• W1920473032 cites W1543910225 @default.
• W1920473032 cites W1549628832 @default.
• W1920473032 cites W1554042779 @default.
• W1920473032 cites W1587087111 @default.
• W1920473032 cites W158928715 @default.
• W1920473032 cites W1591339234 @default.
• W1920473032 cites W1600427338 @default.
• W1920473032 cites W1835526604 @default.
• W1920473032 cites W1876153904 @default.
• W1920473032 cites W1892943724 @default.
• W1920473032 cites W1946436666 @default.
• W1920473032 cites W1964564641 @default.
• W1920473032 cites W1966274615 @default.
• W1920473032 cites W1968131914 @default.
• W1920473032 cites W1973828738 @default.
• W1920473032 cites W1974169246 @default.
• W1920473032 cites W1979510313 @default.
• W1920473032 cites W1980080388 @default.
• W1920473032 cites W1980749588 @default.
• W1920473032 cites W1986177591 @default.
• W1920473032 cites W1986296119 @default.
• W1920473032 cites W1986954508 @default.
• W1920473032 cites W1987100877 @default.
• W1920473032 cites W1988179473 @default.
• W1920473032 cites W1993916511 @default.
• W1920473032 cites W1997685015 @default.
• W1920473032 cites W2001569101 @default.
• W1920473032 cites W2001622142 @default.
• W1920473032 cites W2002565017 @default.
• W1920473032 cites W2003224117 @default.
• W1920473032 cites W2005673005 @default.
• W1920473032 cites W2008247440 @default.
• W1920473032 cites W2009667219 @default.
• W1920473032 cites W2010355095 @default.
• W1920473032 cites W2012175961 @default.
• W1920473032 cites W2012329129 @default.
• W1920473032 cites W2012868581 @default.
• W1920473032 cites W2013534902 @default.
• W1920473032 cites W2015763420 @default.
• W1920473032 cites W2018404032 @default.
• W1920473032 cites W2018641902 @default.
• W1920473032 cites W2019408299 @default.
• W1920473032 cites W2019435777 @default.
• W1920473032 cites W2020446261 @default.
• W1920473032 cites W2020817345 @default.
• W1920473032 cites W2023416205 @default.
• W1920473032 cites W2023682483 @default.
• W1920473032 cites W2023727546 @default.
• W1920473032 cites W2023804289 @default.
• W1920473032 cites W2025364863 @default.
• W1920473032 cites W2027855259 @default.
• W1920473032 cites W2030191770 @default.
• W1920473032 cites W2031434196 @default.
• W1920473032 cites W2031613996 @default.
• W1920473032 cites W2031724434 @default.
• W1920473032 cites W2032070007 @default.
• W1920473032 cites W2034574190 @default.
• W1920473032 cites W2035024583 @default.
• W1920473032 cites W2038884679 @default.
• W1920473032 cites W2042039516 @default.
• W1920473032 cites W2042820647 @default.
• W1920473032 cites W2044783794 @default.
• W1920473032 cites W2045084108 @default.
• W1920473032 cites W2046079214 @default.
• W1920473032 cites W2046389521 @default.
• W1920473032 cites W2047136706 @default.
• W1920473032 cites W2047500483 @default.
• W1920473032 cites W2050671627 @default.
• W1920473032 cites W2051766330 @default.
• W1920473032 cites W2052250424 @default.
• W1920473032 cites W2052571887 @default.
• W1920473032 cites W2055622229 @default.
• W1920473032 cites W2059804455 @default.
• W1920473032 cites W2059995303 @default.
• W1920473032 cites W2062344492 @default.
• W1920473032 cites W2064189557 @default.
• W1920473032 cites W2064365233 @default.
• W1920473032 cites W2064634791 @default.
• W1920473032 cites W2064654478 @default.
• W1920473032 cites W2065044742 @default.
• W1920473032 cites W2066488678 @default.
• W1920473032 cites W2067580682 @default.
• W1920473032 cites W2067849514 @default.
• W1920473032 cites W2067923136 @default.
• W1920473032 cites W2068968931 @default.
• W1920473032 cites W2069055935 @default.
• W1920473032 cites W2069745890 @default.
• W1920473032 cites W2073584573 @default.
• W1920473032 cites W2077802087 @default.
• W1920473032 cites W2079894038 @default.

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