FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1921390505> ?p ?o ?g. }

• W1921390505 endingPage "2680" @default.
• W1921390505 startingPage "2675" @default.
• W1921390505 abstract "The rate-limiting step in diphtheria toxin (DT) intoxication of Vero cells has been determined utilizing cycloheximide as an inhibitor of the intoxication process. Cycloheximide is shown to inhibit the toxin catalyzed ADP-ribosylation of elongation factor 2 (EF-2). The inhibition is blocked by puromycin thus establishing the ribosome as the location of cycloheximide protection. Washing cells free of cycloheximide rapidly reverses the protective effect. The initial rates of protein synthesis inhibition observed after removal of cycloheximide from DT-intoxicated cells are 5 to 12-fold greater than rates observed in unprotected cells and are shown to reflect ADP-ribosylation of EF-2 by cytosolic DT. Ten to thirty minutes after cycloheximide removal, the rate of protein synthesis inhibition abruptly changes to values identical to those of unprotected cells. Both the initial rates and extent of the initial rapid inactivation are directly related to toxin concentration and time of incubation with DT in the presence of cycloheximide. We concluded that: the rate-limiting step in protein synthesis inhibition by DT is not the ADP-ribosylation of EF-2 by cytosolic toxin but rather the earlier entry step of DT into the cytosol. DT enters the cytosol as a bolus of sufficient size to rapidly inactivate all EF-2 in that cell. It is inferred from 1 and 2 that the first order inactivation rate exhibited by DT is the result of the probability of the release of a bolus of toxin to the cytosol of any cell in the population per unit time. Autoradiographic analysis of intoxicated cell populations support this two-population state model. The size of a single bolus or quantum of DT is calculated from data over the range of 10(-11) to 10(-9) M DT and is found to remain constant. We suggest that the cytosolic entry mechanism of DT results from a unique ability of the internalized toxin molecules to destabilize the vesicular membrane resulting in a random release of a bolus of toxin into the cytosol. Because the bolus size remains constant over a 50-fold change in receptor occupancy the possibility is raised that DT undergoes a post-receptor packaging process, package size remaining a constant and package number increasing with receptor occupancy." @default.
• W1921390505 created "2016-06-24" @default.
• W1921390505 creator A5020278909 @default.
• W1921390505 creator A5077719675 @default.
• W1921390505 date "1985-03-01" @default.
• W1921390505 modified "2023-09-27" @default.
• W1921390505 title "Quantal entry of diphtheria toxin to the cytosol." @default.
• W1921390505 cites W1269163945 @default.
• W1921390505 cites W1487184957 @default.
• W1921390505 cites W1543969063 @default.
• W1921390505 cites W1547646332 @default.
• W1921390505 cites W1567594253 @default.
• W1921390505 cites W1642007459 @default.
• W1921390505 cites W1933755140 @default.
• W1921390505 cites W1982074683 @default.
• W1921390505 cites W1982273826 @default.
• W1921390505 cites W2014364051 @default.
• W1921390505 cites W2020072455 @default.
• W1921390505 cites W2030482992 @default.
• W1921390505 cites W2035277465 @default.
• W1921390505 cites W2041193908 @default.
• W1921390505 cites W2053153019 @default.
• W1921390505 cites W2068629712 @default.
• W1921390505 cites W2096490954 @default.
• W1921390505 cites W2117286629 @default.
• W1921390505 cites W2133290481 @default.
• W1921390505 cites W2137333901 @default.
• W1921390505 cites W2164227469 @default.
• W1921390505 cites W2299913891 @default.
• W1921390505 cites W4247478626 @default.
• W1921390505 doi "https://doi.org/10.1016/s0021-9258(18)89414-5" @default.
• W1921390505 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3972800" @default.
• W1921390505 hasPublicationYear "1985" @default.
• W1921390505 type Work @default.
• W1921390505 sameAs 1921390505 @default.
• W1921390505 citedByCount "34" @default.
• W1921390505 countsByYear W19213905052021 @default.
• W1921390505 countsByYear W19213905052022 @default.
• W1921390505 crossrefType "journal-article" @default.
• W1921390505 hasAuthorship W1921390505A5020278909 @default.
• W1921390505 hasAuthorship W1921390505A5077719675 @default.
• W1921390505 hasBestOaLocation W19213905051 @default.
• W1921390505 hasConcept C159047783 @default.
• W1921390505 hasConcept C181199279 @default.
• W1921390505 hasConcept C185592680 @default.
• W1921390505 hasConcept C22070199 @default.
• W1921390505 hasConcept C2777367657 @default.
• W1921390505 hasConcept C2780572238 @default.
• W1921390505 hasConcept C33057221 @default.
• W1921390505 hasConcept C55493867 @default.
• W1921390505 hasConcept C86803240 @default.
• W1921390505 hasConcept C98539663 @default.
• W1921390505 hasConceptScore W1921390505C159047783 @default.
• W1921390505 hasConceptScore W1921390505C181199279 @default.
• W1921390505 hasConceptScore W1921390505C185592680 @default.
• W1921390505 hasConceptScore W1921390505C22070199 @default.
• W1921390505 hasConceptScore W1921390505C2777367657 @default.
• W1921390505 hasConceptScore W1921390505C2780572238 @default.
• W1921390505 hasConceptScore W1921390505C33057221 @default.
• W1921390505 hasConceptScore W1921390505C55493867 @default.
• W1921390505 hasConceptScore W1921390505C86803240 @default.
• W1921390505 hasConceptScore W1921390505C98539663 @default.
• W1921390505 hasIssue "5" @default.
• W1921390505 hasLocation W19213905051 @default.
• W1921390505 hasOpenAccess W1921390505 @default.
• W1921390505 hasPrimaryLocation W19213905051 @default.
• W1921390505 hasRelatedWork W120782368 @default.
• W1921390505 hasRelatedWork W1539194431 @default.
• W1921390505 hasRelatedWork W1680659237 @default.
• W1921390505 hasRelatedWork W1933755140 @default.
• W1921390505 hasRelatedWork W1998434332 @default.
• W1921390505 hasRelatedWork W2002732501 @default.
• W1921390505 hasRelatedWork W2020236362 @default.
• W1921390505 hasRelatedWork W2402306048 @default.
• W1921390505 hasRelatedWork W4307764993 @default.
• W1921390505 hasRelatedWork W81498169 @default.
• W1921390505 hasVolume "260" @default.
• W1921390505 isParatext "false" @default.
• W1921390505 isRetracted "false" @default.
• W1921390505 magId "1921390505" @default.
• W1921390505 workType "article" @default.