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• W1921569590 endingPage "187" @default.
• W1921569590 startingPage "178" @default.
• W1921569590 abstract "Tim23p is a mitochondrial inner membrane protein essential for the import of proteins from the cytosol. Tim23p contains an amino-terminal hydrophilic segment and a carboxyl-terminal hydrophobic domain (Tim23Cp). To study the functions and interactions of the two parts of Tim23p separately, we constructed tim23N, encoding only the hydrophilic region of Tim23p, and tim23C, encoding only the hydrophobic domain of Tim23p. Only the Tim23C protein is imported into mitochondria, indicating that the mitochondrial targeting information in Tim23p resides in its membrane spans or intervening loops. Tim23Cp, however, cannot substitute for full-length Tim23p, suggesting that the hydrophilic portion of Tim23p also performs an essential function in mitochondrial protein import. We found that overexpression of Tim23Cp is toxic to yeast cells that carry the tim23-1 mutation. Excess Tim23Cp causes Tim23-1p to disappear, leavingtim23-1 cells without a full-length version of the Tim23 protein. If Tim17p, another inner membrane import component, is overexpressed along with Tim23Cp, the toxicity of Tim23Cp is largely reversed and the Tim23-1 protein no longer disappears. In coimmunoprecipitations from solubilized mitochondria, Tim17p associates with the Tim23C protein. In addition, we show that Tim23p and Tim17p can be chemically cross-linked to each other in intact mitochondria. We conclude that the hydrophobic domain encoded by tim23Ctargets Tim23p to the mitochondria and mediates the direct interaction between Tim23p and Tim17p. In contrast, Tim23Cp cannot be coimmunoprecipitated with Tim23p, raising the possibility that the hydrophobic domain of Tim23p does not interact with other Tim23 molecules." @default.
• W1921569590 created "2016-06-24" @default.
• W1921569590 creator A5062295531 @default.
• W1921569590 creator A5070810705 @default.
• W1921569590 creator A5073926028 @default.
• W1921569590 date "1998-01-01" @default.
• W1921569590 modified "2023-10-17" @default.
• W1921569590 title "Characterization of the Mitochondrial Inner Membrane Translocase Complex: the Tim23p Hydrophobic Domain Interacts with Tim17p but Not with Other Tim23p Molecules" @default.
• W1921569590 cites W111354659 @default.
• W1921569590 cites W119688228 @default.
• W1921569590 cites W14391843 @default.
• W1921569590 cites W1489541545 @default.
• W1921569590 cites W1497875229 @default.
• W1921569590 cites W1517812132 @default.
• W1921569590 cites W1522668252 @default.
• W1921569590 cites W1543378549 @default.
• W1921569590 cites W1588568344 @default.
• W1921569590 cites W168784921 @default.
• W1921569590 cites W1717988203 @default.
• W1921569590 cites W172411893 @default.
• W1921569590 cites W179325704 @default.
• W1921569590 cites W1806909533 @default.
• W1921569590 cites W1840016496 @default.
• W1921569590 cites W1873754044 @default.
• W1921569590 cites W190131086 @default.
• W1921569590 cites W1964651523 @default.
• W1921569590 cites W1966813371 @default.
• W1921569590 cites W1969737313 @default.
• W1921569590 cites W1972908831 @default.
• W1921569590 cites W1980751552 @default.
• W1921569590 cites W1983790844 @default.
• W1921569590 cites W1984354326 @default.
• W1921569590 cites W1985291517 @default.
• W1921569590 cites W1992271142 @default.
• W1921569590 cites W1994616607 @default.
• W1921569590 cites W1997653705 @default.
• W1921569590 cites W1998916083 @default.
• W1921569590 cites W2001248597 @default.
• W1921569590 cites W2002012495 @default.
• W1921569590 cites W2007195150 @default.
• W1921569590 cites W2008274778 @default.
• W1921569590 cites W2009502549 @default.
• W1921569590 cites W2012941238 @default.
• W1921569590 cites W2021595960 @default.
• W1921569590 cites W2028858498 @default.
• W1921569590 cites W2033409591 @default.
• W1921569590 cites W2034176931 @default.
• W1921569590 cites W2034277598 @default.
• W1921569590 cites W2037464535 @default.
• W1921569590 cites W2040409664 @default.
• W1921569590 cites W2043813249 @default.
• W1921569590 cites W2046929906 @default.
• W1921569590 cites W2050717506 @default.
• W1921569590 cites W2050741966 @default.
• W1921569590 cites W2051338661 @default.
• W1921569590 cites W2060399663 @default.
• W1921569590 cites W2063088638 @default.
• W1921569590 cites W2067310224 @default.
• W1921569590 cites W2070324964 @default.
• W1921569590 cites W2074312777 @default.
• W1921569590 cites W2085058894 @default.
• W1921569590 cites W2087499467 @default.
• W1921569590 cites W2087843741 @default.
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• W1921569590 cites W2100837269 @default.
• W1921569590 cites W2101011253 @default.
• W1921569590 cites W2108116425 @default.
• W1921569590 cites W2120418365 @default.
• W1921569590 cites W2126398452 @default.
• W1921569590 cites W2148397104 @default.
• W1921569590 cites W2151195732 @default.
• W1921569590 cites W2152285702 @default.
• W1921569590 cites W2155055049 @default.
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• W1921569590 cites W2164688332 @default.
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• W1921569590 cites W2169333620 @default.
• W1921569590 cites W28074342 @default.
• W1921569590 cites W43717001 @default.
• W1921569590 cites W63436471 @default.
• W1921569590 cites W941572373 @default.
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• W1921569590 doi "https://doi.org/10.1128/mcb.18.1.178" @default.
• W1921569590 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/121472" @default.
• W1921569590 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9418865" @default.
• W1921569590 hasPublicationYear "1998" @default.
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