FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1921730143> ?p ?o ?g. }

• W1921730143 endingPage "4118" @default.
• W1921730143 startingPage "4111" @default.
• W1921730143 abstract "Chancroid is a sexually transmitted genital ulcer disease caused by Haemophilus ducreyi. Previously, we developed diagnostic DNA probes for H. ducreyi (L. M. Parsons, M. Shayegani, A. L. Waring, and L. H. Bopp, J. Clin. Microbiol. 27:1441-1445, 1989). In the present study, DNA sequencing of one of the diagnostic probes revealed two adjacent open reading frames (ORFs). These H. ducreyi ORFs and the encoded proteins show significant homology with the groE genes and GroES and GroEL heat shock proteins from several bacterial pathogens and with conserved eukaryotic 60-kDa heat shock proteins. The first H. ducreyi ORF (groES) is preceded by sequences similar to those of the Escherichia coli consensus heat shock promoters and is 288 nucleotides long and is capable of encoding a protein of 10.3 kDa. The second ORF (groEL) is 1,641 nucleotides long and is capable of encoding a protein of 57.8 kDa. Northern (RNA blot) analysis demonstrated the presence of a high level of groE mRNA in exponential-phase H. ducreyi grown in hemin broth at the organism's optimal growth temperature (33 degrees C), with increased levels seen following heat shock. Heat shock also increased the thermostability of the organisms, since stressed cells were more resistant to the lethal effects of rapid chilling. Electrophoretic analysis and immunoblots demonstrated that the predominant protein produced by exponential-phase H. ducreyi was a heat-inducible, immunoreactive protein of approximately 60 kDa (GroEL). Also, H. ducreyi groE mRNA and GroEL were expressed and inducible by heat in E. coli. This is the first report describing the cloning, sequencing, and expression of H. ducreyi protein-encoding genes." @default.
• W1921730143 created "2016-06-24" @default.
• W1921730143 creator A5038852972 @default.
• W1921730143 creator A5059707514 @default.
• W1921730143 creator A5086656869 @default.
• W1921730143 date "1992-10-01" @default.
• W1921730143 modified "2023-09-23" @default.
• W1921730143 title "Molecular analysis of the Haemophilus ducreyi groE heat shock operon" @default.
• W1921730143 cites W1585794944 @default.
• W1921730143 cites W1589156898 @default.
• W1921730143 cites W1675592513 @default.
• W1921730143 cites W1778633979 @default.
• W1921730143 cites W1794036758 @default.
• W1921730143 cites W1803554356 @default.
• W1921730143 cites W1830131811 @default.
• W1921730143 cites W1874499737 @default.
• W1921730143 cites W1880802239 @default.
• W1921730143 cites W1941268961 @default.
• W1921730143 cites W1954689247 @default.
• W1921730143 cites W1975722145 @default.
• W1921730143 cites W1984831405 @default.
• W1921730143 cites W1993027574 @default.
• W1921730143 cites W2002151232 @default.
• W1921730143 cites W2009310436 @default.
• W1921730143 cites W2012175055 @default.
• W1921730143 cites W2022721580 @default.
• W1921730143 cites W2027724869 @default.
• W1921730143 cites W2028079049 @default.
• W1921730143 cites W2073242178 @default.
• W1921730143 cites W2088867009 @default.
• W1921730143 cites W2094350294 @default.
• W1921730143 cites W2100773761 @default.
• W1921730143 cites W2104270203 @default.
• W1921730143 cites W2105700720 @default.
• W1921730143 cites W2110338533 @default.
• W1921730143 cites W2120003784 @default.
• W1921730143 cites W2131320751 @default.
• W1921730143 cites W2161680446 @default.
• W1921730143 cites W2162559297 @default.
• W1921730143 cites W2164103857 @default.
• W1921730143 cites W2173097565 @default.
• W1921730143 cites W2191648007 @default.
• W1921730143 cites W2482927381 @default.
• W1921730143 cites W2484671257 @default.
• W1921730143 doi "https://doi.org/10.1128/iai.60.10.4111-4118.1992" @default.
• W1921730143 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/257442" @default.
• W1921730143 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1356926" @default.
• W1921730143 hasPublicationYear "1992" @default.
• W1921730143 type Work @default.
• W1921730143 sameAs 1921730143 @default.
• W1921730143 citedByCount "23" @default.
• W1921730143 countsByYear W19217301432014 @default.
• W1921730143 crossrefType "journal-article" @default.
• W1921730143 hasAuthorship W1921730143A5038852972 @default.
• W1921730143 hasAuthorship W1921730143A5059707514 @default.
• W1921730143 hasAuthorship W1921730143A5086656869 @default.
• W1921730143 hasBestOaLocation W19217301431 @default.
• W1921730143 hasConcept C104317684 @default.
• W1921730143 hasConcept C153911025 @default.
• W1921730143 hasConcept C167625842 @default.
• W1921730143 hasConcept C203075996 @default.
• W1921730143 hasConcept C205260736 @default.
• W1921730143 hasConcept C2776925733 @default.
• W1921730143 hasConcept C2777154767 @default.
• W1921730143 hasConcept C2777587943 @default.
• W1921730143 hasConcept C2780530800 @default.
• W1921730143 hasConcept C46522908 @default.
• W1921730143 hasConcept C47289529 @default.
• W1921730143 hasConcept C501593827 @default.
• W1921730143 hasConcept C54355233 @default.
• W1921730143 hasConcept C547475151 @default.
• W1921730143 hasConcept C86803240 @default.
• W1921730143 hasConcept C87190427 @default.
• W1921730143 hasConcept C89423630 @default.
• W1921730143 hasConceptScore W1921730143C104317684 @default.
• W1921730143 hasConceptScore W1921730143C153911025 @default.
• W1921730143 hasConceptScore W1921730143C167625842 @default.
• W1921730143 hasConceptScore W1921730143C203075996 @default.
• W1921730143 hasConceptScore W1921730143C205260736 @default.
• W1921730143 hasConceptScore W1921730143C2776925733 @default.
• W1921730143 hasConceptScore W1921730143C2777154767 @default.
• W1921730143 hasConceptScore W1921730143C2777587943 @default.
• W1921730143 hasConceptScore W1921730143C2780530800 @default.
• W1921730143 hasConceptScore W1921730143C46522908 @default.
• W1921730143 hasConceptScore W1921730143C47289529 @default.
• W1921730143 hasConceptScore W1921730143C501593827 @default.
• W1921730143 hasConceptScore W1921730143C54355233 @default.
• W1921730143 hasConceptScore W1921730143C547475151 @default.
• W1921730143 hasConceptScore W1921730143C86803240 @default.
• W1921730143 hasConceptScore W1921730143C87190427 @default.
• W1921730143 hasConceptScore W1921730143C89423630 @default.
• W1921730143 hasIssue "10" @default.
• W1921730143 hasLocation W19217301431 @default.
• W1921730143 hasLocation W19217301432 @default.
• W1921730143 hasOpenAccess W1921730143 @default.
• W1921730143 hasPrimaryLocation W19217301431 @default.
• W1921730143 hasRelatedWork W1489792956 @default.
• W1921730143 hasRelatedWork W1921730143 @default.

• next